Cis-3-hexenyl acetate (cis-HXAC) is a major green leaf volatile released by plants, and its atmospheric degradation by O₃ may play an important role in the formation of secondary organic aerosol (SOA). In this study, indoor smog chamber experiments were carried out to investigate the effects of particle acidity and SO₂ on the formation of secondary organic aerosols (SOA) from the ozonolysis of cis-HXAC. Particle formation and growth were characterized using a scanning mobility particle sizer, and the chemical composition of the particles was analyzed by thermal-desorption gas chromatography mass spectrometry, with the main products and reaction pathways of cis-HXAC ozonolysis being identified. The results revealed that acidic seed particles inhibited SOA formation compared to non-acidic seed particles, and SO₂ was considered to play a catalytic role in cis-HXAC ozonolysis, showing a good positive correlation with SOA generation. The results of this study provide new information on the ozonolysis of cis-HXAC, confirm the important role of anthropogenic pollution, and help to further elucidate the potential of green leaf volatiles ozonolysis to generate SOA under complex pollution conditions.