Phytochemical investigation of the volatile oil of Yueju (YJVO) and its constituent herbs induced the detection of 52 compounds in YJVO, mainly monoterpenes and sesquiterpenes as well as a small amount of aromatic and aliphatic compounds. 5 of these compounds were found only in the YJVO instead of the volatile oil of its constituent herbs. The anti-depressant effect of YJVO was proved by behavioral tests in chronic unpredictable mild stress (CUMS) mice. An acute oral toxicity evaluation determined the LD₅₀ of YJVO was 5.780 mL/kg. Doppler ultrasound and laser speckle imaging have detected that the YJVO could improve depression-related cerebral blood flow. In addition, related neurotransmitters and proteins were analyzed through targeted metabolomics and immunofluorescence. The potential antidepressant mechanisms of YJVO related to significantly decreasing Glu in CUMS mice by up-regulating the ERK/AKT-mediated expression of GLT-1.

对越橘挥发油及其组成药材进行植物化学研究，共检测出52种化合物，主要是单萜和倍半萜，还有少量芳香族和脂肪族化合物。其中 5 种化合物仅在 YJVO 中发现，而不是在其组成草药的挥发油中发现。YJVO 的抗抑郁作用通过慢性不可预测轻度应激 (CUMS) 小鼠的行为测试得到证实。急性口服毒性评估确定 YJVO 的 LD₅₀为 5.780 mL/kg。多普勒超声和激光散斑成像检测发现，YJVO 可以改善与抑郁症相关的脑血流量。此外，还通过靶向代谢组学和免疫荧光分析了相关的神经递质和蛋白质。YJVO 的潜在抗抑郁机制与通过上调 ERK/AKT 介导的 GLT-1 表达显着降低 CUMS 小鼠的 Glu 水平有关。