微生物脱乙酰酶的性质及其生物技术应用,Applied Microbiology and Biotechnology

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微生物脱乙酰酶的性质及其生物技术应用
Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2023-06-16 , DOI: 10.1007/s00253-023-12613-1
Shuping Zou _{1,

2} , Xia Li _{1,

2} , Yinfeng Huang _{1,

2} , Bing Zhang _{1,

2} , Heng Tang _{1,

2} , Yaping Xue _{1,

2} , Yuguo Zheng _{1,

2}

Affiliation

摘要

脱乙酰酶是一类能够催化乙酰化底物水解去除乙酰基的酶，用于生产各种高质量的产品，是最具影响力的工业酶之一。这些酶是高度特异性、无毒、可持续且环保的生物催化剂。脱乙酰酶和脱乙酰化合物已广泛应用于制药、医学、食品和环境中。本文综合总结了脱乙酰酶的来源、特征、分类和应用。此外，还总结了不同微生物来源的脱乙酰酶的典型结构特征。我们还回顾了脱乙酰酶催化的各种脱乙酰化合物的反应，例如壳寡糖（COS）、菌硫醇、7-氨基头孢菌酸（7-ACA）、葡萄糖胺、氨基酸和多胺。旨在阐述脱乙酰酶在工业应用中的优势和挑战。此外，它还为获得用于酶促脱乙酰化的有前途和创新的生物催化剂提供了前景。

关键点

• 介绍了各种微生物的微生物脱乙酰酶的基本特性。

• 总结了微生物脱乙酰酶的生化特征、结构和催化机制。

• 讨论了微生物脱乙酰酶在食品、制药、医学和环境中的应用。

"点击查看英文标题和摘要"

Properties and biotechnological applications of microbial deacetylase

Abstract

Deacetylases, a class of enzymes that can catalyze the hydrolysis of acetylated substrates to remove the acetyl group, used in producing various products with high qualities, are one of the most influential industrial enzymes. These enzymes are highly specific, non-toxic, sustainable, and eco-friendly biocatalysts. Deacetylases and deacetylated compounds have been widely applicated in pharmaceuticals, medicine, food, and the environment. This review synthetically summarizes deacetylases’ sources, characterizations, classifications, and applications. Moreover, the typical structural characteristics of deacetylases from different microbial sources are summarized. We also reviewed the deacetylase-catalyzed reactions for producing various deacetylated compounds, such as chitosan-oligosaccharide (COS), mycothiol, 7-aminocephalosporanic acid (7-ACA), glucosamines, amino acids, and polyamines. It is aimed to expound on the advantages and challenges of deacetylases in industrial applications. Moreover, it also serves perspectives on obtaining promising and innovative biocatalysts for enzymatic deacetylation.