A Multifunctional Nanozyme with NADH Dehydrogenase-Like Activity and Nitric Oxide Release under Near-Infrared Light Irradiation as an Efficient Therapeutic for Antimicrobial Resistance Infection and Wound Healing,Advanced Healthcare Materials

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A Multifunctional Nanozyme with NADH Dehydrogenase-Like Activity and Nitric Oxide Release under Near-Infrared Light Irradiation as an Efficient Therapeutic for Antimicrobial Resistance Infection and Wound Healing
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2023-06-16 , DOI: 10.1002/adhm.202300568
Yi Wang ₁ , Hong-Dong Shi ₂ , Hai-Lin Zhang ₁ , Yu- Yu Chen ₃ , Bing Ren ₁ , Qi Tang ₁ , Qi Sun ₄ , Qian-Ling Zhang ₃ , Jin-Gang Liu ₁

Affiliation

In recent years, antimicrobial resistance (AMR) has become one of the greatest threats to human health. There is an urgent need to develop new antibacterial agents to effectively treat AMR infection. Herein, a novel nanozyme platform (Cu,N-GQDs@Ru-NO) is prepared, where Cu,N-doped graphene quantum dots (Cu,N-GQDs) are covalently functionalized with a nitric oxide (NO) donor, ruthenium nitrosyl (Ru-NO). Under 808 nm near-infrared (NIR) light irradiation, Cu,N-GQDs@Ru-NO demonstrates nicotinamide adenine dinucleotide (NADH) dehydrogenase-like activity for photo-oxidizing NADH to NAD⁺, thus disrupting the redox balance in bacterial cells and resulting in bacterial death; meanwhile, the onsite NIR light-delivered NO effectively eradicates the methicillin-resistant Staphylococcus aureus (MRSA) bacterial and biofilms, and promotes wound healing; furthermore, the nanozyme shows excellent photothermal effect that enhances the antibacterial efficacy as well. With the combination of NADH dehydrogenase activity, photothermal therapy, and NO gas therapy, the Cu,N-GQDs@Ru-NO nanozyme displays both in vitro and in vivo excellent efficacy for MRSA infection and biofilm eradication, which provides a new therapeutic modality for effectively treating MRSA inflammatory wounds.

中文翻译：

具有 NADH 脱氢酶样活性和近红外光照射下释放一氧化氮的多功能纳米酶可有效治疗抗菌素耐药性感染和伤口愈合

近年来，抗菌素耐药性（AMR）已成为人类健康的最大威胁之一。迫切需要开发新的抗菌药物来有效治疗AMR感染。在此，制备了一种新型纳米酶平台（Cu,N-GQDs@Ru-NO），其中Cu,N掺杂石墨烯量子点（Cu,N-GQDs）与一氧化氮（NO）供体亚硝酰基钌共价功能化(Ru-NO)。在 808 nm 近红外 (NIR) 光照射下，Cu,N-GQDs@Ru-NO 表现出烟酰胺腺嘌呤二核苷酸 (NADH) 脱氢酶样活性，可将 NADH 光氧化为 NAD + ，从而破坏细菌细胞中的氧化还原^平衡，导致细菌死亡；同时，现场近红外光照射的NO有效消灭耐甲氧西林金黄色葡萄球菌（MRSA）细菌和生物膜，促进伤口愈合；此外，纳米酶表现出优异的光热效应，也增强了抗菌功效。Cu,N-GQDs@Ru-NO纳米酶结合NADH脱氢酶活性、光热疗法和NO气体疗法，在体外和体内对MRSA感染和生物膜根除表现出优异的功效，为MRSA感染和生物膜根除提供了新的治疗方式。有效治疗MRSA炎症伤口。

更新日期：2023-06-16

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