用于修复感染骨缺损的双功能水凝胶集成 3D 打印支架,ACS Biomaterials Science & Engineering

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用于修复感染骨缺损的双功能水凝胶集成 3D 打印支架
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2023-06-15 , DOI: 10.1021/acsbiomaterials.3c00564
Qianqian Zhang ₁ , Xiaojun Zhou ₁ , Haibo Du ₁ , Yujie Ha ₁ , Yao Xu ₂ , Rongguang Ao ₃ , Chuanglong He ₁

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由于感染与骨缺损的共存，感染性骨缺损的临床治疗难度大、耗时长，同时控制感染和修复骨缺损被认为是一种有前途的治疗方法。在这项研究中，通过将三维（3D）打印支架与水凝胶相结合来制造双药物输送支架系统，用于修复感染的骨缺损。3D打印的聚己内酯支架与含有小分子药物芬戈莫德（FTY720）的可生物降解介孔二氧化硅纳米粒子结合在一起，以提供结构支撑并促进血管生成和成骨。以乙醛透明质酸（AHA）和羧甲基壳聚糖（NOCC）为原料，通过席夫碱反应制备万古霉素（Van）负载水凝胶，可填充3D打印支架的孔隙，形成双功能复合支架。体外结果表明，复合支架具有 Van 浓度依赖性抗菌特性。此外，装载FTY720的复合支架在体外表现出优异的生物相容性、血管化和成骨能力。在细菌感染的大鼠股骨缺损模型中，与其他组相比，双药复合支架在感染控制和骨再生方面均表现出更好的结果。因此，所制备的双功能复合支架在感染性骨缺损的治疗中具有潜在的应用前景。

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Bifunctional Hydrogel-Integrated 3D Printed Scaffold for Repairing Infected Bone Defects

The clinical treatment of infectious bone defects is difficult and time-consuming due to the coexistence of infection and bone defects, and the simultaneous control of infection and repair of bone defects is considered a promising therapy. In this study, a dual-drug delivery scaffold system was fabricated by the combination of a three-dimensional (3D) printed scaffold with hydrogel for infected bone defects repair. The 3D printed polycaprolactone scaffold was incorporated with biodegradable mesoporous silica nanoparticles containing the small molecular drug fingolimod (FTY720) to provide structural support and promote angiogenesis and osteogenesis. The vancomycin (Van)-loaded hydrogel was prepared from aldehyde hyaluronic acid (AHA) and carboxymethyl chitosan (NOCC) by the Schiff base reaction, which can fill the pores of the 3D-printed scaffold to produce a bifunctional composite scaffold. The in vitro results demonstrated that the composite scaffold had Van concentration-dependent antimicrobial properties. Furthermore, the FTY720-loaded composite scaffold demonstrated excellent biocompatibility, vascularization, and osteogenic ability in vitro. In the rat femoral defect model with bacterial infection, the dual-drug composite scaffold showed a better outcome in both infection control and bone regeneration compared to other groups. Therefore, the prepared bifunctional composite scaffold has potential application in the treatment of infected bone defects.

更新日期：2023-06-15

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