基因组学、代谢组学、傅里叶变换红外技术在腐败大眼金枪鱼腐败希瓦氏菌腐败目标评价中的应用,Journal of Agricultural and Food Chemistry

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基因组学、代谢组学、傅里叶变换红外技术在腐败大眼金枪鱼腐败希瓦氏菌腐败目标评价中的应用
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-06-12 , DOI: 10.1021/acs.jafc.3c02113
Xin-Yun Wang _{1,

2,

3,

4} , Jun Yan _{1,

2,

3,

4} , Jing Xie _{1,

2,

3,

4}

Affiliation

腐败希瓦氏菌是一种典型的腐败菌，常见于海鲜中，具有很高的腐败潜力。然而，腐败希瓦氏菌在基因和代谢水平上的腐败机制尚未得到很好的阐明。这项工作通过基因组测序、代谢组学和傅里叶变换红外 (FTIR) 分析确定了腐败大眼金枪鱼中腐败希瓦氏菌XY07的腐败目标。腐败希瓦氏菌XY07含有一些腐败调节基因：cys基因、his基因、spe基因和rpoS分别在基因组水平上参与硫代谢、组氨酸代谢、精氨酸和脯氨酸降解以及生物膜形成的基因。鉴定出一些腐败基因，如speC、cysM、trxB基因。此外，ABC转运蛋白、精氨酸和脯氨酸代谢；β-丙氨酸代谢；甘氨酸、丝氨酸和苏氨酸代谢；组氨酸代谢；硫代谢；脂类代谢被认为是水产食品腐败过程中的重要途径，通过代谢组学分析揭示了腐烂链球菌XY 07的氨基酸降解功能。l的代谢物-鸟氨酸、5-氨基戊酸和4-氨基丁醛可进一步代谢为亚精胺和精胺，产生腐败气味，并参与精氨酸和脯氨酸代谢，作为腐败调节代谢的关键。因此，腐败希瓦氏菌XY07 被应用于基因组学、代谢组学分析和 FTIR，为腐败目标的研究提供全面的见解。

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Applications of Genomics, Metabolomics, Fourier Transform Infrared in the Evaluation of Spoilage Targets of Shewanella putrefaciens from Spoiled Bigeye Tuna

Shewanella putrefaciens is a typical spoiler that is commonly found in seafood and has high spoilage potential. However, the spoilage mechanism against Shewanella putrefaciens at the gene and metabolism levels has not been well elucidated. This work determined the spoilage targets on Shewanella putrefaciens XY07 from spoiled bigeye tuna by genome sequencing, metabolomics, and Fourier transform infrared (FTIR) analysis. Shewanella putrefaciens XY07 contained some genes on spoilage regulating of cys genes, his genes, spe genes and rpoS gene involved in sulfur metabolism, histidine metabolism, arginine and proline degradation, and biofilm formation at the genome level, respectively. Some spoilage genes like speC, cysM, trxB genes were identified. In addition, ABC transporters, arginine and proline metabolism; beta-alanine metabolism; glycine, serine, and threonine metabolism; histidine metabolism; sulfur metabolism; and lipid metabolism were identified as important pathways related to aquatic food during spoilage, which indicated the functions of amino acid degradation in S. putrefaciens XY 07 by metabolomics analysis. The metabolites of l-ornithine, 5-aminopentanoate, and 4-aminobutyraldehyde could be further metabolized to spermidine and spermine, producing a spoilage odor, and were involved in arginine and proline metabolism serving as key spoilage regulating metabolisms. Therefore, Shewanella putrefaciens XY07 was applied to genomics, metabolomics analysis, and FTIR to provide comprehensive insight into the investigation of spoilage targets.

更新日期：2023-06-12

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