The 4-ethylphenyl sulfate (4-EPS) is considered a well-known gut microbiota derived metabolite that is involved in various human diseases and ailments. Thus, in the present work, an electrochemical sensor was developed using a polydopamine (PDA) based molecularly imprinted polymer (MIP) for the detection of 4-EPS, a gut metabolite using cyclic voltammetry (CV) for the first time. The synthesized PDA-MIP was characterized by fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) techniques. The PDA-MIP was drop casted on the surface of screen-printed carbon electrode (SPCE) to fabricate an electrochemical sensor. The developed PDA-MIP/SPCE sensor showed a broad linear detection range of 0.1–300 ng/mL (correlation, R² = 0.982) with a high sensitivity of 153.88 μA (log ng/mL)⁻¹cm⁻² and a low limit of detection (LOD) of 0.018 ng/mL. The selectivity studies were also performed against the possible interfering compounds in human urine. The reproducibility of fabricated PDA-MIP/SPCE shows relative standard deviation (RSD) of 5.34%. For demonstrating the effectiveness of the sensor, the real sample analysis was performed in human urine and it shows the good recovery range of 98.1–121.8% with a RSD of 1.35–13.93%. Therefore, the MIP based electrochemical sensor is economically feasible, which may propose a new diagnostic method for identifying diseases associated with the concentration of 4-EPS in other body fluids.

4-乙基苯基硫酸盐 (4-EPS) 被认为是一种众所周知的肠道微生物衍生代谢物，与多种人类疾病有关。因此，在目前的工作中，使用基于聚多巴胺（PDA）的分子印迹聚合物（MIP）开发了一种电化学传感器，首次使用循环伏安法（CV）检测4-EPS（一种肠道代谢物）。通过傅里叶变换红外光谱 (FTIR) 和扫描电子显微镜 (SEM) 技术对合成的 PDA-MIP 进行了表征。将 PDA-MIP 滴铸在丝网印刷碳电极（SPCE）的表面上以制造电化学传感器。开发的 PDA-MIP/SPCE 传感器表现出 0.1–300 ng/mL 的宽线性检测范围（相关性，R ²  = 0.982），灵敏度高达 153.88 μA（log ng/mL）⁻¹ cm ⁻²和检测下限 (LOD) 0.018 ng/mL。还针对人尿液中可能的干扰化合物进行了选择性研究。制造的 PDA-MIP/SPCE 的再现性显示相对标准偏差 (RSD) 为 5.34%。为了证明传感器的有效性，对人体尿液进行了真实样品分析，结果显示回收率良好，为 98.1-121.8%，RSD 为 1.35-13.93%。因此，基于MIP的电化学传感器在经济上是可行的，这可能为识别与其他体液中4-EPS浓度相关的疾病提供一种新的诊断方法。