Microbial electrochemical system autotrophic denitrification has attracted much attention due to its cost-efficiency and clean advantages. The autotrophic denitrification rate highly depends on the input electrons to the cathode. In this study, agricultural waste corncob was filled into sandwich structure anode as low-cost carbon source for electron production. The COMSOL software was used to guide the construction of sandwich structure anode to control carbon source release and enhance electron collection, including suitable pore size (4 mm) and current collector arrangement (five branches). Optimized sandwich structure anode system with the help of 3D printing obtained a higher denitrification efficiency (21.79 ± 0.22 gNO₃^--N/m³d) than anodic systems without pore and current collector. Statistical analysis showed that enhanced autotrophic denitrification efficiency was the responsible for enhanced denitrification performance of the optimized anode system. This study provides a strategy to improve the autotrophic denitrification performance of the microbial electrochemical system by optimizing the anode structure.

微生物电化学系统自养反硝化因其成本效益和清洁优势而备受关注。自养反硝化速率很大程度上取决于阴极的输入电子。在这项研究中，将农业废弃玉米芯填充到三明治结构阳极中作为电子生产的低成本碳源。COMSOL软件用于指导三明治结构阳极的构建，以控制碳源释放并增强电子收集，包括合适的孔径（4毫米）和集电器排列（五个分支）。借助3D打印优化的夹层结构阳极系统获得了更高的脱氮效率（21.79±0.22 gNO ₃ ^- -N/m ³d) 优于没有孔和集电器的阳极系统。统计分析表明，自养反硝化效率的提高是优化阳极系统反硝化性能增强的原因。本研究提供了一种通过优化阳极结构来提高微生物电化学系统自养反硝化性能的策略。