Advanced precision agriculture requires the objective measurement of the structural and functional properties of plants. Biochemical profiles in leaves can differ depending on plant growing conditions. By quantitatively detecting these changes, farm production processes can be optimized to achieve high-yield, high-quality, and nutrient dense agricultural products. To enable the rapid and non-destructive detection on site, this study demonstrates the development of a new custom-designed portable handheld Vis–NIR spectrometer that collects leaf reflectance spectra, wirelessly transfers the spectral data through Bluetooth, and provides both raw spectral data and processed information. The spectrometer has two preprogramed methods: anthocyanin and chlorophyll quantification. Anthocyanin content of red and green lettuce estimated with the new spectrometer showed an excellent correlation coefficient of 0.84 with those determined by a destructive gold standard biochemical method. The differences in chlorophyll content were measured using leaf senescence as a case study. Chlorophyll Index calculated with the handheld spectrometer gradually decreased with leaf age as chlorophyll degrades during the process of senescence. The estimated chlorophyll values were highly correlated with those obtained from a commercial fluorescence-based chlorophyll meter with a correlation coefficient of 0.77. The developed portable handheld Vis–NIR spectrometer could be a simple, cost-effective, and easy to operate tool that can be used to non-invasively monitor plant pigment and nutrient content efficiently.

先进的精准农业需要客观测量植物的结构和功能特性。叶子的生化特征可能因植物生长条件而异。通过定量检测这些变化，可以优化农业生产过程，以获得高产、优质、营养丰富的农产品。为了实现快速、无损的现场检测，本研究展示了一种新型定制设计的便携式手持式可见-近红外光谱仪的开发，该光谱仪收集叶子反射光谱，通过蓝牙无线传输光谱数据，并提供原始光谱数据和处理过的信息。光谱仪有两种预编程方法：花青素和叶绿素定量。使用新型光谱仪估算的红生菜和绿生菜的花青素含量与破坏性金标准生化方法测定的花青素含量显示出良好的相关系数，达到 0.84。使用叶子衰老作为案例研究来测量叶绿素含量的差异。手持式光谱仪计算的叶绿素指数随着叶龄的增长而逐渐下降，因为叶绿素在衰老过程中降解。估计的叶绿素值与商业荧光叶绿素计获得的值高度相关，相关系数为 0.77。所开发的便携式手持式可见-近红外光谱仪是一种简单、经济高效且易于操作的工具，可用于有效地非侵入性监测植物色素和营养成分。