Molecular Genetics, Microbiology and Virology ( IF 0.4 ) Pub Date : 2023-06-11 , DOI: 10.3103/s089141682301010x
S. V. Vasilieva , N. V. Alekseeva , Yu. M. Romanova , A. F. Vanin
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Abstract
The structure of nitric oxide (NO) donors, paramagnetic dinitrosyl iron complexes with SH ligands (DNICs), contains two nitrosyl groups, NO and NO+, which are released from a DNIC upon degradation in the cell. The selective ability of diethyl dithiocarbamate (DETC) to bind NO was used in this work to study the functions of nitrosonium cation NO+ in the regulation of biofilm formation by nitric oxide donors. The combined treatment of Pseudomonas aeruginosa with DETC and NO donors, sodium nitrite NaNO2 or binuclear DNIC with glutathione (B-DNIChglu), multiply reduced the biofilm formation relative to the control and single treatments with each compound. The biofilm formation depended on the ratio of components. At a tenfold excess of DETC against DNICglu, the formation rate decreased by three times, and under a fivefold excess, by 1.8 times. The stable [Fe2+–DETC] complex formed during the combined treatment functioned as an NO trap, leading to the block in the synthesis of the signaling regulator DNICglu from NO and iron and to the accumulation of nitrosonium cation NO+ in the cell. The maximum decrease in the biofilm formation rate was established in the option with successive treatment of cells with DETC with a lag period of 40 min after the introduction of the NaNO2 donor and the formation in DNIC with thiosulfate ligands, universal NO signaling molecules of all biosystems, in the cells. The results obtained expand knowledge about the functions of nitrosonium cation and contribute to the disclosure of mechanisms of the toxic activity of DNIC donors. They correlate with our earlier results on NO+ inhibition of E. coli colony formation in experiments with DNICglu, as well as with the data of other researchers obtained on yeast and in MCF7 cancer cell culture.
中文翻译:
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亚硝基阳离子 NO+ 抑制一氧化氮调节铜绿假单胞菌生物膜形成的功能
摘要
一氧化氮 (NO) 供体的结构是顺磁性二亚硝基铁与 SH 配体 (DNIC) 的配合物,包含两个亚硝基基团 NO 和 NO +,它们在细胞中降解时从 DNIC 中释放出来。本工作利用二乙基二硫代氨基甲酸酯 (DETC) 结合 NO 的选择性能力来研究亚硝鎓阳离子 NO +在一氧化氮供体调节生物膜形成中的功能。与对照和每种化合物的单一处理相比,用DETC和NO供体、亚硝酸钠NaNO 2或双核DNIC与谷胱甘肽(B-DNIChglu)联合处理铜绿假单胞菌,成倍减少了生物膜的形成。生物膜的形成取决于组分的比例。当 DETC 相对于 DNICglu 过量十倍时,形成速率降低了三倍,而当过量五倍时,形成速率降低了 1.8 倍。联合处理过程中形成的稳定的[Fe 2+ -DETC]复合物起到NO陷阱的作用,导致NO和铁合成信号调节剂DNICglu的受阻以及亚硝鎓阳离子NO +在细胞内的积累。生物膜形成率的最大降低是在引入 NaNO 2供体后用 DETC 连续处理细胞并在 40 分钟内用硫代硫酸盐配体(所有通用 NO 信号分子)在 DNIC 中形成的滞后期确定的。生物系统,在细胞中。获得的结果扩展了有关亚硝鎓阳离子功能的知识,并有助于揭示 DNIC 供体的毒性活性机制。它们与我们早期在 DNICglu 实验中 NO +抑制大肠杆菌集落形成的结果以及其他研究人员在酵母和 MCF7 癌细胞培养物中获得的数据相关。