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Light-activated carbon dot nanozyme with scandium for a highly efficient and pH-universal bio-nanozyme cascade colorimetric assay
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2023-06-12 , DOI: 10.1039/d3tb00927k Xueshan Chen 1 , Yao Lin 2 , Jing Liao 3 , Jinyi Zhang 1 , Chengbin Zheng 1
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2023-06-12 , DOI: 10.1039/d3tb00927k Xueshan Chen 1 , Yao Lin 2 , Jing Liao 3 , Jinyi Zhang 1 , Chengbin Zheng 1
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Nanozyme-based colorimetric assays have attracted much attention due to their cost-effectiveness, high stability, and sensitivity. In particular, the catalytic cascade imparted by the biological enzyme is highly selective. However, developing an efficient, one-pot, and pH-universal bio-nanozyme cascade remains challenging. Considering the tunable activity of the photo-activated nanozyme, we herein demonstrated a pH-universal colorimetric assay based on the Sc3+-boosted photocatalytic oxidation of carbon dots (C-dots). As a strong Lewis acid, Sc3+ shows ultra-fast complexation with OH− over a broad pH range and dramatically decreases the pH of the buffer solutions. In addition to regulating the pH, Sc3+ also binds to the C-dots to produce a persistent and strongly oxidizing intermediate based on photo-induced electron transfer. The proposed Sc3+-boosted photocatalytic system was successfully used in a cascade colorimetric assay with biological enzymes for assessing their activity as well as the detection of enzyme inhibitors at neutral and alkaline pH. Instead of designing new nanozymes for catalytic cascades, this work suggests that introducing promoters can be a convenient strategy in practical applications.
中文翻译：
含钪的光活化碳点纳米酶可用于高效、pH 通用的生物纳米酶级联比色测定
基于纳米酶的比色测定因其成本效益、高稳定性和灵敏度而备受关注。特别地，生物酶所产生的催化级联具有高度选择性。然而，开发一种高效、一锅式、pH 通用的生物纳米酶级联仍然具有挑战性。考虑到光激活纳米酶的可调活性，我们在此展示了基于Sc 3+增强的碳点（C-点）光催化氧化的pH通用比色测定。作为强路易斯酸，Sc 3+在较宽的 pH 范围内与 OH -表现出超快的络合作用，并显着降低缓冲溶液的 pH 值。除了调节 pH 值外，Sc 3+还与碳点结合，基于光诱导电子转移产生持久且强氧化的中间体。所提出的 Sc 3+增强光催化系统已成功用于生物酶的级联比色测定，以评估其活性以及在中性和碱性 pH 值下检测酶抑制剂。这项工作表明，引入启动子可能是实际应用中的一种便捷策略，而不是设计用于催化级联的新纳米酶。
更新日期：2023-06-12
中文翻译：
含钪的光活化碳点纳米酶可用于高效、pH 通用的生物纳米酶级联比色测定
基于纳米酶的比色测定因其成本效益、高稳定性和灵敏度而备受关注。特别地，生物酶所产生的催化级联具有高度选择性。然而，开发一种高效、一锅式、pH 通用的生物纳米酶级联仍然具有挑战性。考虑到光激活纳米酶的可调活性，我们在此展示了基于Sc 3+增强的碳点（C-点）光催化氧化的pH通用比色测定。作为强路易斯酸，Sc 3+在较宽的 pH 范围内与 OH -表现出超快的络合作用，并显着降低缓冲溶液的 pH 值。除了调节 pH 值外，Sc 3+还与碳点结合，基于光诱导电子转移产生持久且强氧化的中间体。所提出的 Sc 3+增强光催化系统已成功用于生物酶的级联比色测定，以评估其活性以及在中性和碱性 pH 值下检测酶抑制剂。这项工作表明，引入启动子可能是实际应用中的一种便捷策略，而不是设计用于催化级联的新纳米酶。
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