<br>基于肽组学的牛奶光氧化评价,Food Research International

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基于肽组学的牛奶光氧化评价
Food Research International ( IF 7.0 ) Pub Date : 2023-06-10 , DOI: 10.1016/j.foodres.2023.113113
Sijia Tan ₁ , Qingyang Zhang ₁ , Xiaoyan Pei ₂ , Dongfei Tan ₃ , Can Guo ₁ , Sumeng Chen ₁ , Gang Chen ₄

Affiliation

光氧化是加工和销售过程中牛奶质量恶化的主要原因之一。本研究旨在使用肽组学技术研究光氧化后肽的变化，以及奶牛物种、氧含量和光强度如何影响光氧化。使用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）对不同的肽进行鉴定和定量。对 18 个牛奶样品进行光处理。7 种类型的肽被鉴定为光氧化标志物。随后，研究了牛奶品种、氧含量和光照强度对光氧化的影响，并进行了感官评价。奶牛品种、氧含量和光照强度都会影响光氧化。感官评价证实，光和氧是牛奶光氧化所必需的。肽 m/z+ 529.2783 （LLDEIKEVV）在不同品种的牛奶和不同品牌的市售牛奶中均表现出较大的多重性变化，其含量与氧和光密切相关。这种肽在无氧和无光下不产生，其相对含量随着光照的持续时间而增加。这些结果表明，肽组学方法是区分正常牛奶和光氧化牛奶的有效工具。

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Evaluation of milk photooxidation based on peptidomics

Photooxidation is one of the main causes of the deterioration of milk quality during processing and marketing. This study aimed to investigate the variation in peptides after photooxidation using peptidomic techniques, and how cow species, oxygen content, and light intensity affect photooxidation. The different peptides were identified and quantified using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Eighteen milk samples were subjected to light treatment. Seven types of peptides were identified as photooxidation markers. Subsequently, the effects of milk variety, oxygen content, and light intensity on photooxidation were studied, and sensory evaluations were performed. Dairy cow breed, oxygen content, and light intensity all affect photooxidation. Sensory evaluation verified that light and oxygen are necessary for the photooxidation of milk. The peptide m/z+ 529.2783 (LLDEIKEVV), both in different varieties of milk and in different brands of commercially available milk, showed a large variation in multiplicity, and its content was closely related to oxygen and light. This peptide was not produced in the absence of oxygen and light, and its relative content increased with the duration of light exposure. These results suggest that the peptidomics method is an effective tool for distinguishing between normal and photooxidized milk.

更新日期：2023-06-10

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