This study aimed to investigate the physiological and biochemical responses of filamentous microalga Tribonema minus to different Na₂SeO₃ concentrations and its selenium absorption and metabolism to evaluate the potential in treating selenium-containing wastewater. The results showed that low Na₂SeO₃ concentrations promoted growth by increasing chlorophyll content and antioxidant capacity, whereas high concentrations caused oxidative damage. Although Na₂SeO₃ exposure reduced lipid accumulation compared with the control, it significantly increased carbohydrate, soluble sugar, and protein contents, with the highest carbohydrate productivity of 117.97 mg/L/d at 0.5 mg/L Na₂SeO₃. Furthermore, this alga effectively absorbed Na₂SeO₃ in the growth medium and converted most of it into volatile selenium and a small part into organic selenium (predominantly as selenocysteine), showing strong selenite removal efficacy. This is the first report on the potential of T. minus to produce valuable biomass while removing selenite, providing new insights into the economic feasibility of bioremediation of selenium-containing wastewater.

本研究旨在研究丝状微藻Tribonema minus对不同Na ₂ SeO ₃浓度的生理生化反应及其硒的吸收和代谢，以评价其处理含硒废水的潜力。结果表明，低Na ₂ SeO ₃浓度通过增加叶绿素含量和抗氧化能力来促进生长，而高浓度则会造成氧化损伤。尽管Na ₂ SeO ₃与对照相比，暴露减少了脂质积累，显着增加了碳水化合物、可溶性糖和蛋白质含量，在0.5 mg/L Na 2 SeO 3 条件下碳水化合物生产率最高，为117.97 mg/ _L / _d。此外，该藻有效吸收生长介质中的Na ₂ SeO _{3 ，并将其大部分转化为挥发性硒，小部分转化为有机硒（主要为硒代半胱氨酸），表现出很强的亚硒酸盐去除效果。}这是第一份关于T. minus在去除亚硒酸盐的同时产生有价值的生物质的潜力的报告，为含硒废水生物修复的经济可行性提供了新的见解。