Soil organic matter plays an important role in the stability, transport, and fate of soil colloids. At present, studies have mostly focused on the effects of adding exogenous organic matter on soil colloidal properties, while there is very limited research on the effect of reduced inherent soil organic matter on the environmental behavior of soil colloids. This study investigated the stability and transport behavior of black soil colloids (BSC) and black soil colloids with reduced inherent organic matter (BSC-ROM) under different ionic strength (5, 50 mM) and background solution pH (4.0, 7.0, and 9.0) conditions. Meanwhile, the release behavior of two soil colloids in the saturated sand column under transient ionic strength conditions was also studied. The results showed that both ionic strength reduction and pH increase increased the negative charges of BSC and BSC-ROM, and improved the electrostatic repulsion between soil colloids and grain surface, thereby promoting the stability and mobility of soil colloids. The decrease in inherent organic matter had little effect on the surface charge of soil colloids, suggesting that the electrostatic repulsion was not the main force affecting the stability and mobility of BSC and BSC-ROM, and reducing inherent organic matter might significantly reduce the stability and mobility of soil colloids by weakening the steric hindrance interaction. The decrease of transient ionic strength reduced the depth of the energy minimum and activated the soil colloids retained on the surface of the grain at three pH conditions. This study is helpful to predict the potential impact of soil organic matter degradation on the fate of BSC in natural environment system.