酰肼连接的富含钛的多孔有机聚合物的自组装用于有效富集人血清中的糖肽和磷酸肽,Analyst

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酰肼连接的富含钛的多孔有机聚合物的自组装用于有效富集人血清中的糖肽和磷酸肽
Analyst ( IF 3.6 ) Pub Date : 2023-06-09 , DOI: 10.1039/d3an00709j
Danni Wang ₁ , Quanshou Feng ₁ , Yiting Luo ₁ , Weimin Wang ₁ , Yinghua Yan ₁ , Chuan-Fan Ding ₁

Affiliation

本工作以肼、2,3-二羟基对苯二甲醛（DHTA）和1,3,5-苯三甲酸三甲酯（TP）为配体，合成了富钛酰肼连接的多孔有机聚合物（酰肼-POPs-Ti ⁴⁺ ）。Hydrazide-POPs-Ti ⁴⁺与 HILIC 和 IMAC 结合可用于同时富集糖肽和磷酸肽。该方案的检测限为糖肽 0.1 fmol μL ⁻¹和 0.005 fmol μL ⁻¹对于磷酸肽，对于糖肽和磷酸肽的选择性分别为1:1000和1:2000。对于实际生物样品分析，从健康人血清中选择性地捕获了与129种糖蛋白相关的201种糖肽和与21种磷蛋白相关的26种磷酸肽，并且在乳腺癌血清中富集了与117种糖蛋白相关的186种糖肽和与50种磷蛋白相关的60种磷酸肽。患者。基因本体分析表明，所鉴定的糖蛋白和磷蛋白与乳腺癌相关，包括补体成分C1q与低密度脂蛋白颗粒的结合、蛋白质氧化和补体激活，表明这些相关通路可能参与了乳腺癌的疾病病理学。癌症。

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Self-assembly of hydrazide-linked porous organic polymers rich in titanium for efficient enrichment of glycopeptides and phosphopeptides from human serum

In this work, titanium-rich hydrazide-linked porous organic polymers (hydrazide-POPs-Ti⁴⁺) were synthesized using hydrazine, 2,3-dihydroxyterephthalaldehyde (DHTA) and trimethyl 1,3,5-benzenetricarboxylate (TP) as the ligands. Hydrazide-POPs-Ti⁴⁺ combined with HILIC and IMAC can be used for simultaneous enrichment of glycopeptides and phosphopeptides. The detection limit of this protocol is 0.1 fmol μL⁻¹ for glycopeptides and 0.005 fmol μL⁻¹ for phosphopeptides, and the selectivities are 1 : 1000 and 1 : 2000 for glycopeptides and phosphopeptides, respectively. For practical bio-sample analysis, 201 glycopeptides associated with 129 glycoproteins and 26 phosphopeptides associated with 21 phosphoproteins were selectively captured from healthy human serum, and 186 glycopeptides associated with 117 glycoproteins and 60 phosphopeptides associated with 50 phosphoproteins were enriched in the serum of breast cancer patients. Gene Ontology analysis indicated that the identified glycoproteins and phosphoproteins were linked to breast cancer, including the binding of complement component C1q and low-density lipoprotein particles, protein oxidation and complement activation, suggesting that these connected pathways are probably engaged in the disease pathology of breast cancer.

更新日期：2023-06-09

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