Pathogenic bacteria are associated with high morbidity rates and present significant diagnostic challenges in terms of rapid detection. This study introduces a magnetic separation-based electrochemical biosensor for the detection of Methicillin-resistant Staphylococcus aureus (MRSA). Vancomycin (Van) was used to modify on the surface of polyethyleneimine (PEI) mediated MBs (MBs-PEI-Van) for separation and enrichment of MRSA. The MBs-PEI-Van shown a satisfactory stability and applicability with capture effective (CE) > 85% in both PBS and cerebrospinal fluid (CSF) samples. MXene@Au with controllable size of AuNPs was synthesized by a self-reduction method and employed to modify the glassy carbon electrode (GCE). Immunoglobulin G (IgG) was loaded onto the modified electrode to immobilize MRSA, and ferroceneboronic acid (Fc-BA) was used as a probe for quantitative determination. The differential pulse voltammetry (DPV) current was plotted against the concentration of MRSA from 3.8 × 10¹ to 3.8 × 10⁷ CFU/mL with a limit of detection (LOD) of 3.8 × 10¹ CFU/mL. In addition, MRSA was successfully detected in spiked CSF samples with satisfactory recoveries (94.35–107.81 %) and validation results (RSD < 11 %). Overall, this study presents a promising method for the detection of MRSA, with the potential to be further developed into a universal pathogen detection method.

致病菌与高发病率相关，并且在快速检测方面提出了重大的诊断挑战。本研究介绍了一种基于磁分离的电化学生物传感器，用于检测耐甲氧西林金黄色葡萄球菌(MRSA)。使用万古霉素（Van）对聚乙烯亚胺（PEI）介导的MB（MBs-PEI-Van）表面进行修饰，用于分离和富集MRSA。MBs-PEI-Van 在 PBS 和脑脊液 (CSF) 样品中表现出令人满意的稳定性和适用性，捕获有效 (CE) > 85%。通过自还原法合成了纳米金尺寸可控的MXene@Au，并用于修饰玻碳电极（GCE）。将免疫球蛋白G（IgG）负载到修饰电极上固定MRSA，并使用二茂铁硼酸（Fc-BA）作为探针进行定量测定。微分脉冲伏安法 (DPV) 电流相对于 MRSA 浓度绘制，从 3.8 × 10 ¹到 3.8 × 10 ⁷ CFU/mL，检测限 (LOD) 为 3.8 × 10 ¹ CFU/mL。此外，在加标的脑脊液样本中成功检测到了 MRSA，回收率 (94.35–107.81%) 和验证结果令人满意 (RSD < 11%)。总体而言，这项研究提出了一种很有前景的 MRSA 检测方法，并有可能进一步发展成为一种通用的病原体检测方法。