Determination of 1,3-Diphenylguanidine, 1,3-Di-o-tolylguanidine, and 1,2,3-Triphenylguanidine in Human Urine Using Liquid Chromatography-Tandem Mass Spectrometry,Environmental Science & Technology

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Determination of 1,3-Diphenylguanidine, 1,3-Di-o-tolylguanidine, and 1,2,3-Triphenylguanidine in Human Urine Using Liquid Chromatography-Tandem Mass Spectrometry
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2023-06-08 , DOI: 10.1021/acs.est.3c00412
Zhong-Min Li _{1,

2} , Kurunthachalam Kannan _{1,

2}

Affiliation

1,3-Diphenylguanidine (DPG), 1,3-di-o-tolylguanidine (DTG), and 1,2,3-triphenylguanidine (TPG) are rubber additives widely present in the indoor environment. Nevertheless, little is known about their human exposure. We developed a method for the quantification of DPG, DTG, and TPG in human urine, using high-performance liquid chromatography-tandem mass spectrometry. The quantitative analysis of target analytes at parts-per-trillion levels in urine was optimized using hydrophilic–lipophilic balanced solid-phase extraction and isotopic dilution. The method limits of detection and quantification were in the range of 0.002–0.02 and 0.005–0.05 ng/mL, respectively. The recoveries of all analytes in human urine fortified at 1, 5, 10, and 20 ng/mL concentrations were in the range of 75.3–111%, with standard deviations of 0.7–4%. The repeated measurement of similarly fortified human urine yielded intra-day and inter-day variations of 0.47–3.90 and 0.66–3.76%, respectively. The validated method was applied in the measurement of DPG, DTG, and TPG in real human urine samples, which revealed the occurrence of DPG in children’s urine samples (n = 15) with a detection frequency of 73% and at a median concentration of 0.05 ng/mL. DPG was found in 20% of adults’ urine samples (n = 20).

中文翻译：

液相色谱-串联质谱法测定人尿液中的 1,3-二苯胍、1,3-二邻甲苯基胍和 1,2,3-三苯胍

1,3-二苯胍 (DPG), 1,3-二邻-甲苯胍（DTG）和1,2,3-三苯基胍（TPG）是广泛存在于室内环境中的橡胶添加剂。然而，人们对它们与人类的接触情况知之甚少。我们开发了一种使用高效液相色谱-串联质谱法定量人尿液中 DPG、DTG 和 TPG 的方法。使用亲水亲脂平衡固相萃取和同位素稀释对尿液中万亿分之一水平的目标分析物进行定量分析。检测和定量的方法限分别在0.002-0.02和0.005-0.05 ng/mL范围内。人尿液中所有浓度为 1、5、10 和 20 ng/mL 的强化分析物的回收率在 75.3–111% 范围内，标准偏差为 0.7–4%。对类似强化的人尿的重复测量产生的日内和日间变化分别为 0.47-3.90 和 0.66-3.76%。将经过验证的方法应用于真实人体尿液样本中 DPG、DTG 和 TPG 的测量，揭示了儿童尿液样本中 DPG 的存在（n = 15)，检测频率为 73%，中位浓度为 0.05 ng/mL。20% 的成人尿液样本中发现了 DPG（n = 20）。

更新日期：2023-06-08

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