Abstract

Four amino phases representing 3-aminopropyl silica gel batches with different numbers of grafted functional groups are compared. To evaluate the chromatographic properties of the adsorbents, the Tanaka test for hydrophilic stationary phases is used and the retention of polar substances of various classes in the HILIC (hydrophilic) mode is studied. It is shown that even small changes in the nitrogen content between different batches of 3-aminopropyl silica gel have a significant effect on the retention of polar analytes. The hydrophilicity of a substrate is shown to have the greatest effect on its chromatographic behavior, and its evaluation using the Tanaka test is the basis for selecting a batch to separate specific classes of polar substances or for further modification to obtain new phases.

中文翻译：

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不同氮含量的3-氨丙基二氧化硅在HILIC模式下的性能比较

摘要

比较了代表具有不同数量的接枝官能团的 3-氨基丙基硅胶批次的四个氨基相。为了评估吸附剂的色谱特性，使用了亲水固定相的田中试验，并研究了 HILIC（亲水）模式下各类极性物质的保留情况。结果表明，即使不同批次的 3-氨基丙基硅胶之间氮含量的微小变化也会对极性分析物的保留产生显着影响。底物的亲水性对其色谱行为影响最大，使用田中试验对其进行评估是选择批次来分离特定类别极性物质或进一步改性以获得新相的基础。