通过己二胺介导的交联在 Ti6Al4V 合金上沉积抗氧化剂和细胞相容性咖啡酸基薄膜,ACS Applied Materials & Interfaces

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通过己二胺介导的交联在 Ti6Al4V 合金上沉积抗氧化剂和细胞相容性咖啡酸基薄膜
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-06-08 , DOI: 10.1021/acsami.3c05564
Maria L Alfieri ₁ , Giacomo Riccucci _{2,

3} , Sara Ferraris _{2,

3} , Andrea Cochis ₄ , Alessandro C Scalia ₄ , Lia Rimondini ₄ , Lucia Panzella ₁ , Silvia Spriano _{2,

3} , Alessandra Napolitano ₁

Affiliation

先进骨植入物的一种有前景的方法是在钛表面沉积具有改善治疗性能的有机薄膜。在此，我们报道了通过利用己二胺 (HMDA) 交联能力，在抛光和化学预处理的 Ti6Al4V 合金上有效浸涂沉积基于咖啡酸 (CA) 的薄膜。正如之前研究中报道的那样，涂层的黄/绿色表明了苯扎吖啶系统的形成，这是由于 CA 与 HMDA 的氨基相互作用而形成的。通过 Folin-Ciocalteu 方法、荧光显微镜、水接触角测量、X 射线光电子能谱 (XPS)、zeta 电位测量和傅里叶变换红外光谱对涂层表面进行表征。确认钛表面上存在均匀的涂层。胶带附着力测试还证明了涂层的最佳机械附着力，特别是在化学预处理的基材上。有趣的是，这两种薄膜都表现出显着的抗氧化特性（2,2-二苯基-1-三硝基苯肼和铁降低抗氧化能力测定），这些特性随着时间的推移而持续存在，即使在材料长期储存后也不会损失。涂层在暴露基团方面的特征（XPS 和 zeta 电位滴定证据）显然取决于钛基材的表面预处理。评估了所开发涂层的细胞相容性、清除剂抗氧化活性和抗菌性能。最有希望的结果是化学预处理的 CA/HMDA 涂层表面表现出良好的细胞相容性和高活性氧清除能力，防止其在促炎条件下在细胞内积聚；此外，通过扫描电子显微镜观察到其具有防止 3D 生物膜样细菌聚集体形成的防污作用。这些结果为开发具有天然酚类薄涂层的创新钛表面用于骨接触植入物开辟了新的前景。通过扫描电子显微镜观察到了防止 3D 生物膜样细菌聚集体形成的防污作用。这些结果为开发具有天然酚类薄涂层的创新钛表面用于骨接触植入物开辟了新的前景。通过扫描电子显微镜观察到了防止 3D 生物膜样细菌聚集体形成的防污作用。这些结果为开发具有天然酚类薄涂层的创新钛表面用于骨接触植入物开辟了新的前景。

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Deposition of Antioxidant and Cytocompatible Caffeic Acid-Based Thin Films onto Ti6Al4V Alloys through Hexamethylenediamine-Mediated Crosslinking

A promising approach for advanced bone implants is the deposition on titanium surfaces of organic thin films with improved therapeutic performances. Herein, we reported the efficient dip-coating deposition of caffeic acid (CA)-based films on both polished and chemically pre-treated Ti6Al4V alloys by exploiting hexamethylenediamine (HMDA) crosslinking ability. The formation of benzacridine systems, resulting from the interaction of CA with the amino groups of HMDA, as reported in previous studies, was suggested by the yellow/green color of the coatings. The coated surfaces were characterized by means of the Folin–Ciocalteu method, fluorescence microscopy, water contact angle measurements, X-ray photoelectron spectroscopy (XPS), zeta-potential measurements, and Fourier transform infrared spectroscopy, confirming the presence of a uniform coating on the titanium surfaces. The optimal mechanical adhesion of the coating, especially on the chemically pre-treated substrate, was also demonstrated by the tape adhesion test. Interestingly, both films exhibited marked antioxidant properties (2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays) that persisted over time and were not lost even after prolonged storage of the material. The feature of the coatings in terms of the exposed groups (XPS and zeta potential titration evidence) was apparently dependent on the surface pre-treatment of the titanium substrate. Cytocompatibility, scavenger antioxidant activity, and antibacterial properties of the developed coatings were evaluated. The most promising results were obtained in the case of the chemically pre-treated CA/HMDA-based coated surface that showed good cytocompatibility and high reactive oxygen species’ scavenging ability, preventing their intracellular accumulation under pro-inflammatory conditions; moreover, an anti-fouling effect preventing the formation of 3D biofilm-like bacterial aggregates was observed by scanning electron microscopy. These results open new perspectives for the development of innovative titanium surfaces with thin coatings from naturally occurring phenols for bone contact implants.

更新日期：2023-06-08

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