Polyhedral Au Nanoparticle/MoOx Heterojunction-Enhanced Ultrasensitive Dual-Mode Biosensor for miRNA Detection Combined with a Nonenzymatic Cascade DNA Amplification Circuit,Analytical Chemistry

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Polyhedral Au Nanoparticle/MoOx Heterojunction-Enhanced Ultrasensitive Dual-Mode Biosensor for miRNA Detection Combined with a Nonenzymatic Cascade DNA Amplification Circuit
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-06-06 , DOI: 10.1021/acs.analchem.3c01062
Lin Zhao ₁ , Tiantian Li ₁ , Xinlin Xu ₁ , Yang Xu ₁ , Dongxiang Li ₁ , Weiling Song ₁ , Tianrong Zhan ₁ , Peng He ₁ , Hong Zhou ₁ , Jing-Juan Xu ₂ , Hong-Yuan Chen ₂

Affiliation

A novel homologous surface-enhanced Raman scattering (SERS)-electrochemical (EC) dual-mode biosensor based on a 3D/2D polyhedral Au nanoparticle/MoO_x nanosheet heterojunction (PAMS HJ) and target-triggered nonenzyme cascade autocatalytic DNA amplification (CADA) circuit was constructed for highly sensitive detection of microRNA (miRNA). Mixed-dimensional heterostructures were prepared by in situ growth of polyhedral Au nanoparticles (PANPs) on the surface of MoO_x nanosheets (MoO_x NSs) via a seed-mediated growth method. As a detection substrate, the resulting PAMS HJ shows the synergistic effects of both electromagnetic and chemical enhancements, efficient charge transfer, and robust stability, thus achieving a high SERS enhancement factor (EF) of 4.2 × 10⁹ and strong EC sensing performance. Furthermore, the highly efficient molecular recognition between the target and smart lock probe and the gradually accelerated cascade amplification reaction further improved the selectivity and sensitivity of our sensing platform. The detection limits of miRNA-21 in SERS mode and EC mode were 0.22 and 2.69 aM, respectively. More importantly, the proposed dual-mode detection platform displayed excellent anti-interference and accuracy in the analysis of miRNA-21 in human serum and cell lysates, indicating its potential as a reliable tool in the field of biosensing and clinical analysis.

中文翻译：

用于 miRNA 检测的多面体 Au 纳米粒子/MoOx 异质结增强型超灵敏双模式生物传感器与非酶级联 DNA 扩增电路相结合

一种新型同源表面增强拉曼散射（SERS）-电化学（EC）双模式生物传感器，基于3D/2D多面体Au纳米颗粒/MoO _x纳米片异质结（PAMS HJ）和目标触发非酶级联自催化DNA扩增（CADA）构建了用于高灵敏度检测 microRNA (miRNA) 的电路。通过在MoO _x纳米片 (MoO _xNSs）通过种子介导的生长方法。^{作为检测底物，所得的 PAMS HJ 显示出电磁增强和化学增强的协同效应、高效的电荷转移和鲁棒的稳定性，从而实现了 4.2 × 10 9}的高 SERS 增强因子 (EF)和强大的 EC 传感性能。此外，目标和智能锁探针之间的高效分子识别以及逐渐加速的级联放大反应进一步提高了我们的传感平台的选择性和灵敏度。SERS模式和EC模式下miRNA-21的检测限分别为0.22和2.69 aM。更重要的是，所提出的双模式检测平台在人血清和细胞裂解物中的miRNA-21分析中表现出优异的抗干扰性和准确性，表明其作为生物传感和临床分析领域的可靠工具的潜力。

更新日期：2023-06-06

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