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Synergistic antifungal properties of lauraldehyde and geraniol against Aspergillus flavus in pistachio
Food Control ( IF 5.6 ) Pub Date : 2023-06-06 , DOI: 10.1016/j.foodcont.2023.109915
Yijia Xie , Yifang Cao , Yan Zhang , Fengsong Liu , Hao Xu , Xinglong Xiao

Natural plant-derived compounds have been recognized as promising fungicides for reducing Aspergillus flavus growth in agricultural commodities. In this study, the synergistic antifungal activity of plant-derived compounds against A. flavus growth in stored pistachios was investigated. The minimum inhibitory concentration (MIC) of lauraldehyde, geraniol, dihydrocoumarin, and p-anisaldehyde against A. flavus in potato dextrose broth (PDB) were 0.40, 0.20, 0.40, and 0.80 mg/mL, respectively. Whereas, after screening 6 groups of agents using the checkerboard method, only one combination, i.e., that of lauraldehyde and geraniol (SLG), showed synergistic effect (fractional inhibitory concentration index = 0.5) on A. flavus. In addition, at 2 MIC, SLG completely inhibited the spore germination of A. flavus, colony radial growth, and aflatoxin biosynthesis. At the same time, the mycelial growth was inhibited by more than 98%. Further research found that fumigation with SLG significantly inhibited the growth of A. flavus in stored pistachios and reduced aflatoxin biosynthesis. Moreover, the pistachios with SLG fumigation exhibited higher soluble protein content, lower malonaldehyde (MDA) content, and lower carbonyl values, indicating that fumigation with SLG could effectively delay the trend of quality deterioration in stored pistachios. These results supported SLG as a potential green antifungal agent for the shelf-life extension of stored pistachios.



中文翻译:

月桂醛和香叶醇对开心果中黄曲霉的协同抗真菌特性

天然植物源化合物已被认为是有前途的杀菌剂,可减少农产品中黄曲霉的生长。在这项研究中,研究了植物源化合物对储存开心果中黄曲霉生长的协同抗真菌活性。马铃薯葡萄糖肉汤 (PDB) 中月桂醛、香叶醇、二氢香豆素和对茴香醛对黄曲霉的最低抑菌浓度 (MIC)分别为 0.40、0.20、0.40 和 0.80 mg/mL。而用棋盘法筛选了6组药剂后,只有一种组合,即月桂醛和香叶醇的组合(S LG),对黄曲霉表现出协同效应(分数抑制浓度指数 = 0.5)。此外,在2 MIC时,SLG完全抑制黄曲霉孢子萌发、菌落径向生长和黄曲霉毒素生物合成。同时,菌丝生长被抑制98%以上。进一步研究发现, SLG熏蒸可显着抑制贮藏开心果中黄曲霉的生长,并减少黄曲霉毒素的生物合成。此外,经S LG熏蒸的开心果表现出较高的可溶性蛋白含量、较低的丙二醛(MDA)含量和较低的羰基值,表明经S LG熏蒸的开心果可有效延缓贮藏开心果品质恶化的趋势。这些结果支持 S LG作为一种潜在的绿色抗真菌剂,可延长储存开心果的保质期。

更新日期:2023-06-06
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