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Enhanced phytotoxicity of 4-chloro-3-methyphenol and lindane under sodium and potassium salt stresses
Chemosphere ( IF 8.1 ) Pub Date : 2023-06-06 , DOI: 10.1016/j.chemosphere.2023.139111
Anfei He 1 , Jingyi Feng 1 , Qi Yu 1 , Jing Jiang 1 , Jing Ding 1 , Kun Qian 1 , Haoting Tian 1
Affiliation  

Investigating the uptake of organic pollutants by plants under salt stress is critical for evaluating crop contamination, understanding the mechanism of plant uptake, and implementing phytoremediation. The uptake of a highly phytotoxic contaminant, 4-Chloro-3-Methyphenol (CMP, 45 mg L−1), from solutions by wheat seedling with or without Na+ and K+ was studied to illustrate the synergistic effect of salt on phytotoxicity of CMP, using uptake kinetics, transpiration, Ca2+ leakage and fatty acid saturation as indicators. The influence of Na+ and K+ on the uptake of lindane, a relatively low toxic contaminant, from soil was also explored. Under CMP-Na+ and CMP-K+ exposure, the concentrations of CMP in both root and shoot were lower than those under CMP exposure, as a result of the inhibition of transpiration caused by Na+ and K+ stresses. Low concentration of CMP did not reveal serious toxicity on cell membrane. No apparent difference of MDA generation in root cells was observed, due to the lethal concentration of CMP. The relatively small variation of Ca2+ leakage and fatty acid saturation degree in the root cell under exposure of CMP, CMP-Na+ and CMP-K+, compared to intracellular CMP content, suggested the enhanced phytotoxicity of CMP induced by salt. Higher MDA concentration in shoot cell under CMP-Na+ and CMP-K+ exposure compared with that under CMP exposure again showed the synergetic toxicity of CMP. High Na+ and K+ concentration significantly facilitated the uptake of lindane by wheat seedlings in soils, indicating that it could boost the permeability of cell membrane, thereby increasing the toxicity of linande to wheat seedlings. The short-term effect of low salt concentration on the uptake of lindane was not obvious, but long-term exposure also led to increased uptake. In conclusion, the presence of salt could amplify the phtotoxicity of organic contaminant via several mechanisms.



中文翻译:

钠盐和钾盐胁迫下 4-氯-3-甲基苯酚和林丹的植物毒性增强

研究盐胁迫下植物对有机污染物的吸收对于评估作物污染、了解植物吸收机制和实施植物修复至关重要。研究了小麦幼苗从含有或不含 Na +和 K +的溶液中吸收高植物毒性污染物 4-氯-3-甲基苯酚(CMP,45 mg L -1 )的情况,以说明盐对小麦幼苗植物毒性的协同作用。 CMP,以摄取动力学、蒸腾作用、Ca 2+渗漏和脂肪酸饱和度为指标。还探讨了 Na +和 K +对从土壤中吸收林丹(一种毒性相对较低的污染物)的影响。CMP-Na +下在CMP-K +胁迫和CMP-K + 胁迫下,根和地上部的CMP 浓度均低于CMP 胁迫下的浓度,这是由于Na + 和K +胁迫抑制蒸腾作用的结果。低浓度的 CMP 并未表现出对细胞膜的严重毒性。由于 CMP 的致死浓度,未观察到根细胞中 MDA 生成的明显差异。与细胞内CMP含量相比, CMP、CMP-Na +和CMP-K +暴露下根细胞中Ca 2+渗漏和脂肪酸饱和度的变化相对较小,表明盐诱导CMP的植物毒性增强。CMP-Na +下芽细胞中的 MDA 浓度较高CMP-K +暴露与CMP暴露下的比较再次显示了CMP的协同毒性。高Na +和K +浓度显着促进土壤中小麦幼苗对林丹的吸收,表明其可以增加细胞膜的通透性,从而增加林丹对小麦幼苗的毒性。低盐浓度对林丹摄取的短期影响不明显,但长期暴露也会导致摄取增加。总之,盐的存在可以通过多种机制放大有机污染物的光毒性。

更新日期:2023-06-08
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