Electrochemical immunosensors are widely used in the detection of biomarkers. In order to further improve the detection sensitivity and accuracy, we constructed a dual-signal electrochemical immunosensor with CoTe₂@CeO₂ nanocomposites (CoTe₂@CeO₂ NCs) as an electrocatalytic label and successfully applied it to the detection of prostate-specific antigen (PSA). It was discovered that the CoTe₂@CeO₂ NCs obtained by two-step hydrothermal process with outstanding oxygen evolution reaction (OER) activity and the Te^- oxidation during OER, it produces a strong OER signal and a huge oxidation peak signal in neutral solution. Based on this, we propose an ingenious and simple dual-signal strategy that requires only one label, using CoTe₂@CeO₂ NCs as an electrochemical immunosensor electrocatalytic label. Experimental results show that the developed dual-signal electrochemical immunosensor with a broad linear response (0.002 ∼ 200 ng mL⁻¹) and very low detection limits (I_peak: 8.26 fg mL⁻¹, I_OER: 448 fg mL⁻¹) for PSA. It was successfully used for the detection of real samples with good detection and self-calibration capability. Furthermore, this constructed dual-signal immunosensor may also be used to detect other biomarkers by altering the recognition components. In conclusion, the construction strategy of this novel immunosensor is very meaningful for the early detection and treatment of diseases.

电化学免疫传感器广泛用于生物标志物的检测。为了进一步提高检测灵敏度和准确度，我们构建了一种以CoTe ₂ @CeO ₂纳米复合物(CoTe ₂ @CeO ₂ NCs)为电催化标记物的双信号电化学免疫传感器，并成功应用于前列腺特异性抗原的检测。 （公益广告）。结果发现，两步水热法制备的CoTe ₂ @CeO _{2 NCs 具有优异的析氧反应 (OER) 活性和 Te} ^-在 OER 过程中氧化，它会产生强烈的 OER 信号，在中性溶液中会产生巨大的氧化峰信号。基于此，我们提出了一种巧妙而简单的双信号策略，只需要一个标签，使用 CoTe ₂ @CeO ₂ NCs 作为电化学免疫传感器电催化标签。实验结果表明，所开发的双信号电化学免疫传感器具有宽线性响应（0.002 ∼ 200 ng mL ^-1）和极低的检测限（I _peak：8.26 fg mL ^-1，I _OER：448 fg mL ^-1) 用于 PSA。成功用于实际样品的检测，具有良好的检测和自校准能力。此外，这种构建的双信号免疫传感器也可用于通过改变识别成分来检测其他生物标志物。综上所述，这种新型免疫传感器的构建策略对于疾病的早期检测和治疗具有重要意义。