In the current study, a decahedron-like silver nanostructure (D-AgNs) was successfully created in an aqueous solution in the presence of Polyvinylpyrrolidone (PVP) as a capping agent using the gamma-radiolysis approach without the use of a reducing agent. The synthesized D-AgNs were characterized using various analytical tools such as UV–Vis. spectroscopy, SEM, XRD, HRTEM, EDX and FTIR. UV–Vis. absorption spectra showed considerable surface Plasmon resonance (SPR) bands at 350–600 nm, indicating that colloidal D-AgNs had been successfully synthesized. HRTEM image demonstrates well-dispersed uniformly decahedral shapes that are well separated from each other. The produced nanoparticles were effectively stabilized by PVP through interactions, confirmed by the FTIR anlaysis. The synthesis of D-AgNs using gamma radiation was accomplished, in addition its antimicrobial potential, antibiofilm activity, and the effect of UV rays were assessed. In addition, protein leakage assays and SEM imaging were employed to analyze the antimicrobial reaction’s mechanism. A wide variety of bacteria, including S. aureus, P. aeruginosa, and C. albicans, were deactivated by D-AgNs. In the antibiofilm assay, D-AgNs inhibited the biofilm formation of S. aureus (89.58%), E. coli (80.35%), and P. aureginosa (78.45%). After investigating the effect of D-AgNs on the growth curve of S. aureus, we concluded that D-AgNs affect the growth curve of S. aureus, and the curve was reduced to be 0.125. The formation of holes in the S. aureus cell membrane is explained by the fact that the amount of cellular protein released from the bacteria is directly proportional to the concentration of D-AgNs, which was determined to be 259.25 µg/ml at concentration equal to 1.0 mg/mL.

在当前的研究中，在聚乙烯吡咯烷酮 (PVP) 作为封端剂存在的情况下，使用伽马辐射分解方法在不使用还原剂的情况下，成功地在水溶液中创建了类似十面体的银纳米结构 (D-AgNs)。使用各种分析工具（例如 UV-Vis）对合成的 D-AgN 进行了表征。光谱学、SEM、XRD、HRTEM、EDX 和 FTIR。紫外-可见。吸收光谱在 350-600 nm 处显示出相当大的表面等离子体共振 (SPR) 带，表明已成功合成胶体 D-AgN。HRTEM 图像展示了良好分散的均匀十面体形状，这些形状彼此分离良好。通过 FTIR 分析证实，PVP 通过相互作用有效地稳定了产生的纳米颗粒。完成了使用伽马辐射合成 D-AgNs，此外，还评估了其抗菌潜力、抗生物膜活性和紫外线的影响。此外，还采用了蛋白质渗漏测定和 SEM 成像来分析抗菌反应的机制。种类繁多的细菌，包括金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌被 D-AgN 灭活。在抗生物膜测定中，D-AgNs 抑制金黄色葡萄球菌(89.58%)、大肠杆菌(80.35%) 和金黄色葡萄球菌(78.45%)的生物膜形成。在研究了D-AgNs对金黄色葡萄球菌生长曲线的影响后，我们得出结论，D-AgNs影响了金黄色葡萄球菌的生长曲线，曲线缩小为0.125。金黄色葡萄球菌孔的形成细胞膜被细菌释放的细胞蛋白的量与 D-AgN 的浓度成正比，在浓度等于 1.0 mg/mL 时确定为 259.25 µg/mL。