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The D3-creatine dilution method non-invasively measures muscle mass in mice
Aging Cell ( IF 8.0 ) Pub Date : 2023-06-05 , DOI: 10.1111/acel.13897 Lauren Wimer 1 , Elena Goncharova 1 , Sofiya Galkina 1 , Edna Nyangau 2 , Mahalakshmi Shankaran 2 , Asia Davis 1 , Leandro Prado 1 , Maria Castro Munoz 1 , Sharon Epstein 1 , Cavan Patterson 1 , Nicholas Shaum 3 , Mark Hellerstein 2 , William Evans 2 , Simon Melov 1
Aging Cell ( IF 8.0 ) Pub Date : 2023-06-05 , DOI: 10.1111/acel.13897 Lauren Wimer 1 , Elena Goncharova 1 , Sofiya Galkina 1 , Edna Nyangau 2 , Mahalakshmi Shankaran 2 , Asia Davis 1 , Leandro Prado 1 , Maria Castro Munoz 1 , Sharon Epstein 1 , Cavan Patterson 1 , Nicholas Shaum 3 , Mark Hellerstein 2 , William Evans 2 , Simon Melov 1
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Developing accurate methods to quantify age-related muscle loss (sarcopenia) could greatly accelerate development of therapies to treat muscle loss in the elderly, as current methods are inaccurate or expensive. The current gold standard method for quantifying sarcopenia is dual-energy X-ray absorptiometry (DXA) but does not measure muscle directly—it is a composite measure quantifying “lean mass” (muscle) excluding fat and bone. In humans, DXA overestimates muscle mass, which has led to erroneous conclusions about the importance of skeletal muscle in human health and disease. In animal models, DXA is a popular method for measuring lean mass. However, instrumentation is expensive and is potentially limited by anesthesia concerns. Recently, the D3-creatine (D3Cr) dilution method for quantifying muscle mass was developed in humans and rats. This method is faster, cheaper, and more accurate than DXA. Here, we demonstrate that the D3Cr method is a specific assay for muscle mass in mice, and we test associations with DXA and body weight. We evaluated the D3Cr method compared to DXA-determined lean body mass (LBM) in aged mice and reported that DXA consistently overestimates muscle mass with age. Overall, we provide evidence that the D3Cr dilution method directly measures muscle mass in mice. Combined with its ease of use, accessibility, and non-invasive nature, the method may prove to more quickly advance development of preclinical therapies targeting sarcopenia.
中文翻译：
D3-肌酸稀释法无创测量小鼠肌肉质量
开发准确的方法来量化与年龄相关的肌肉损失（肌肉减少症）可以大大加速治疗老年人肌肉损失的疗法的开发，因为目前的方法不准确或昂贵。目前量化肌肉减少症的金标准方法是双能 X 射线吸收测定法 (DXA)，但并不直接测量肌肉——它是一种量化“去脂质量”（肌肉）的综合测量方法，不包括脂肪和骨骼。对于人类，DXA 高估了肌肉质量，从而导致对骨骼肌在人类健康和疾病中的重要性得出错误的结论。在动物模型中，DXA 是测量瘦体重的常用方法。然而，仪器价格昂贵，并且可能受到麻醉问题的限制。最近，在人类和大鼠中开发了用于量化肌肉质量的 D 3 -肌酸（D 3 Cr）稀释方法。该方法比 DXA 更快、更便宜且更准确。在这里，我们证明 D 3 Cr 方法是一种针对小鼠肌肉质量的特异性测定方法，并且我们测试了 DXA 和体重的关联。我们评估了 D 3 Cr 方法与老年小鼠 DXA 测定的去脂体重 (LBM) 的比较，并报告 DXA 始终高估了随着年龄增长的肌肉质量。总的来说，我们提供了 D 3 Cr 稀释法直接测量小鼠肌肉质量的证据。结合其易用性、可及性和非侵入性，该方法可能会更快地推进针对肌肉减少症的临床前疗法的开发。
更新日期：2023-06-05
中文翻译：
D3-肌酸稀释法无创测量小鼠肌肉质量
开发准确的方法来量化与年龄相关的肌肉损失（肌肉减少症）可以大大加速治疗老年人肌肉损失的疗法的开发，因为目前的方法不准确或昂贵。目前量化肌肉减少症的金标准方法是双能 X 射线吸收测定法 (DXA)，但并不直接测量肌肉——它是一种量化“去脂质量”（肌肉）的综合测量方法，不包括脂肪和骨骼。对于人类，DXA 高估了肌肉质量，从而导致对骨骼肌在人类健康和疾病中的重要性得出错误的结论。在动物模型中，DXA 是测量瘦体重的常用方法。然而，仪器价格昂贵，并且可能受到麻醉问题的限制。最近，在人类和大鼠中开发了用于量化肌肉质量的 D 3 -肌酸（D 3 Cr）稀释方法。该方法比 DXA 更快、更便宜且更准确。在这里，我们证明 D 3 Cr 方法是一种针对小鼠肌肉质量的特异性测定方法，并且我们测试了 DXA 和体重的关联。我们评估了 D 3 Cr 方法与老年小鼠 DXA 测定的去脂体重 (LBM) 的比较，并报告 DXA 始终高估了随着年龄增长的肌肉质量。总的来说，我们提供了 D 3 Cr 稀释法直接测量小鼠肌肉质量的证据。结合其易用性、可及性和非侵入性，该方法可能会更快地推进针对肌肉减少症的临床前疗法的开发。
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