High-Strength Cell Sheets and Vigorous Hydrogels from Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells,ACS Applied Materials & Interfaces

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High-Strength Cell Sheets and Vigorous Hydrogels from Mesenchymal Stem Cells Derived from Human Embryonic Stem Cells
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-06-04 , DOI: 10.1021/acsami.3c03117
Baojie Guo _{1,

2,

3} , Yongchao Duan _{1,

2} , Zhongwen Li _{1,

2,

4} , Yao Tian _{1,

2} , Xuedi Cheng ₅ , Chunxu Liang ₆ , Wenjing Liu ₄ , Bin An _{1,

2} , Wumei Wei _{1,

2} , Tingting Gao _{1,

2} , Shasha Liu _{1,

2} , Xiyuan Zhao _{1,

2} , Shuaishuai Niu _{2,

4,

7} , Chenxin Wang _{1,

2,

4} , Yukai Wang _{1,

2,

3,

4} , Liu Wang _{1,

2,

3,

4} , Guihai Feng _{1,

2,

3,

4} , Wei Li _{1,

2,

3,

4} , Jie Hao _{1,

3,

4,

7} , Qi Gu _{1,

2,

3,

4} , Qi Zhou _{1,

2,

3,

4} , Jun Wu _{1,

2,

4}

Affiliation

Natural cell derivates, including cell sheets (CSs) and matrix gels, have opened new opportunities to probe questions in tissue engineering and regenerative medicine. However, the potential of CSs and hydrogels generated by current protocols is still limited by the challenges of heterogeneity and weak mechanical properties. Here, we developed a 21 day long-term serum-free culture system for human embryonic stem cell (hESC)-derived immunity-and-matrix-regulatory cells (IMRCs). The CSs formed with IMRCs (IMRC-CSs) have a much greater secretion capacity for the extracellular matrix (ECM) and stronger mechanical properties than umbilical cord-derived MSCs, with a ten thousand-fold increase in elastin, a higher elastic modulus of 1500 kPa, a thicker structure of 20.59 μm, and a higher fiber count per square millimeter. The IMRC-CSs could promote corneal chemical injury repair and could be turned into injectable temperature-sensitive hydrogels for uterine adhesion repair via a decellularization process. In summary, we have established a high-strength CS platform using human pluripotent stem cells for the first time, providing a facile and scalable engineering approach for regenerative medicine.

中文翻译：

来自人胚胎干细胞的间充质干细胞的高强度细胞片和活力水凝胶

天然细胞衍生物，包括细胞片 (CS) 和基质凝胶，为探索组织工程和再生医学中的问题开辟了新的机会。然而，当前协议生成的 CSs 和水凝胶的潜力仍然受到异质性和弱机械性能挑战的限制。在这里，我们为人胚胎干细胞 (hESC) 衍生的免疫和基质调节细胞 (IMRC) 开发了一个 21 天的长期无血清培养系统。IMRCs形成的CSs（IMRC-CSs）比脐带来源的MSCs具有更大的细胞外基质（ECM）分泌能力和更强的机械性能，弹性蛋白增加一万倍，弹性模量高达1500 kPa，更厚的结构为 20.59 μm，每平方毫米的纤维数更高。IMRC-CSs 可以促进角膜化学损伤修复，并可以通过脱细胞过程转化为可注射的温度敏感水凝胶，用于子宫粘连修复。总之，我们首次使用人类多能干细胞建立了高强度 CS 平台，为再生医学提供了一种简便且可扩展的工程方法。

更新日期：2023-06-04

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