Co-culture of Kluyveromyces marxianus and Meyerozyma guilliermondii with In Situ Product Recovery of 2-Phenylethanol,Journal of Agricultural and Food Chemistry

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Co-culture of Kluyveromyces marxianus and Meyerozyma guilliermondii with In Situ Product Recovery of 2-Phenylethanol
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-06-05 , DOI: 10.1021/acs.jafc.3c01356
Benedict Ryan Lukito ₁ , Nurhidayah Basri ₁ , Aaron Thong ₁ , Christian Hermansen ₁ , Melanie Weingarten ₁ , Eric Charles Peterson _{1,

2}

Affiliation

Production of 2-phenylethanol (2-PE) via Kluyveromyces marxianus is well-established. However, co-culture with other microbes in combination with in situ product recovery (ISPR) yields improved selectivity and volumetric productivity. Fermentation ofK. marxianus (MUCL 53775) with direct inclusion of absorptive polymer Hytrel3548 achieved ISPR, but accumulation of the byproduct phenylethyl acetate (PEA) was strongly favored. Co-culture of K. marxianus (MUCL 53775) with Meyerozyma guilliermondii (MUCL 28072) with ISPR limited PEA production, thereby improving the 2-PE selectivity from 13 to 90%, compared to a pure culture of K. marxianus (MUCL 53775) under similar conditions. This improved the volumetric productivity by 85% compared to 2-PE ISPR with a pure culture of K. marxianus. This is the first report of co-culture in a two-phase fermentation for 2-PE bioproduction and demonstrates that interactions between co-culture and ISPR techniques can modulate bioproduction between 2-PE and byproduct PEA, and this technique will be explored for other strain combinations and for other high-value molecules of interest.

中文翻译：

马克斯克鲁维酵母和吉利蒙德迈耶罗酵母的共培养与 2-苯乙醇的原位产物回收

通过马克斯克鲁维酵母生产 2-苯乙醇 (2-PE)已经很成熟。然而，与其他微生物的共培养与原位产物回收 (ISPR)相结合可提高选择性和体积生产率。直接包含吸收性聚合物 Hytrel3548 的K. marxianus (MUCL 53775)发酵实现了 ISPR，但强烈支持副产物乙酸苯乙酯 (PEA) 的积累。与K. marxianus的纯培养物相比，K. marxianus (MUCL 53775) 与Meyerozyma guilliermondii (MUCL 28072) 与 ISPR 的共培养限制了 PEA 产量，从而将 2-PE 选择性从 13% 提高到 90%(MUCL 53775) 在类似条件下。与具有K. marxianus纯培养物的 2-PE ISPR 相比，这将容积生产率提高了 85% 。这是关于 2-PE 生物生产的两阶段发酵共培养的第一份报告，表明共培养和 ISPR 技术之间的相互作用可以调节 2-PE 和副产物 PEA 之间的生物生产，该技术将被探索用于其他菌株组合和其他感兴趣的高价值分子。

更新日期：2023-06-05

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