Lipid droplets (LDs) are unique organelles that control the lipid metabolism in cells. It has been identified that the generations of LDs derive from endoplasmic reticulum (ER) and they have closely related with amount of cellular activities for maintaining homeostasis. To further explore the detail interactions between LDs and ER, we have developed a novel polarity-sensitive fluorescent probe LP with distinct D-π-A-π-D framework and applied it to imaging LDs and ER with dual colors at the same time. Probe LP showed well red-shifted emissions with the increase fraction of water in the 1,4- dioxane due to ICT process. In biological imaging, probe LP could visualize LDs and ER with green and red fluorescence separately. Besides, the dynamic behaviors of LDs and ER were achieved using LP during the oleic acids and starvation stimulations. Therefore, probe LP is a valuable molecular tool for investigating the relationships of LDs and ER in various cellular activities.

脂滴 (LD) 是控制细胞脂质代谢的独特细胞器。已经确定LD的世代来源于内质网（ER）并且它们与维持体内平衡的细胞活动量密切相关。为了进一步探索 LD 和 ER 之间的细节相互作用，我们开发了一种具有独特 D-π-A-π-D 框架的新型极性敏感荧光探针LP，并将其应用于双色 LD 和 ER 同时成像。由于 ICT 过程，探针LP显示出随着 1,4-二氧六环中水的比例增加而发生良好的红移排放。在生物成像中，探测LP可以分别用绿色和红色荧光可视化 LD 和 ER。此外，在油酸和饥饿刺激期间使用LP实现了 LD 和 ER 的动态行为。因此，探针LP是一种有价值的分子工具，用于研究 LD 和 ER 在各种细胞活动中的关系。