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Atrazine decontamination by a newly screened psychrotroph Paenarthrobacter sp. KN0901 in an aquatic system: Metabolic pathway, kinetics, and hydroponics experiment
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2023-06-03 , DOI: 10.1016/j.jhazmat.2023.131764
Zhichao Kang 1 , Yang Yang 1 , Chenxu Wang 2 , Yuanyuan Kang 3 , Tianye Wang 4 , Guopeng Zhu 1 , Xuerong Han 5 , Hongwen Yu 4
Affiliation  

Atrazine residues running off the fields and entering water resources are a major threat to food security and the ecosystem. In this study, a psychrotrophic functional strain named KN0901 to remove atrazine residues was screened. KN0901 could degrade 30 mg·L−1 atrazine in 4 days at 15ºC with 105 CFU·mL−1 incubation. The phylogenetic results showed KN0901 belonged to Paenarthrobacter sp. PCR results showed that the functional genes consist of trzN, atzB, and atzC, suggesting atrazine was transformed to cyanuric acid by KN0901. KN0901 could degrade atrazine without adding exogenous carbon and nitrogen sources. What’s more, KN0901 could tolerate extreme low temperature (5ºC) and high atrazine concentration (100 mg·L−1). When growth and degradation curves were compared, the results indicated the length of lag time showed significant correlation to atrazine degradation rate. The hydroponic experiments showed that the toxicity of atrazine was significantly reduced with KN0901 treatment. The study provided an effective, economic, and eco-friendly bioremediation measure to address atrazine contamination.



中文翻译:

新筛选的耐冷菌类杆菌属阿特拉津净化。KN0901 在水生系统中:代谢途径、动力学和水培实验

阿特拉津残留物从田间流失并进入水资源,对粮食安全和生态系统构成重大威胁。本研究筛选了一株具有去除莠去津残留的耐冷功能菌株KN0901。KN0901在15℃、10 5 CFU·mL -1培养下4天内可降解30 mg·L -1阿特拉津。系统发育结果显示KN0901属于Paenarthrobacter sp。PCR结果显示功能基因由trzNatzBatzC组成。,表明莠去津被 KN0901 转化为氰尿酸。KN0901无需添加外源碳源和氮源即可降解莠去津。更重要的是,KN0901可以耐受极低的温度(5℃)和高阿特拉津浓度(100 mg·L -1 )。比较生长曲线和降解曲线,结果表明滞后时间的长度与莠去津的降解率显着相关。水培实验表明,KN0901处理后莠去津的毒性显着降低。该研究为解决莠去津污染提供了一种有效、经济且环保的生物修复措施。

更新日期:2023-06-03
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