<br>BMAC（骨髓抽吸浓缩液）和 PRP（富血小板血浆）联合治疗对 BALB/c 小鼠皮肤伤口愈合的影响,Journal of Burn Care & Research

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BMAC（骨髓抽吸浓缩液）和 PRP（富血小板血浆）联合治疗对 BALB/c 小鼠皮肤伤口愈合的影响
Journal of Burn Care & Research ( IF 1.5 ) Pub Date : 2023-06-02 , DOI: 10.1093/jbcr/irad080
Rebar N Mohammed _{1,

2} , Sadat Abdulla Aziz Sadat ₂ , Snur M A Hassan ₃ , Hawraz Farhad Mohammed ₂ , Derin Omer Ramzi ₄

Affiliation

骨髓是一种柔软的海绵组织，含有间充质干细胞（MSC），因其自我更新性和干性而得到广泛认可。因此，我们假设 BMAC 可能对伤口愈合过程产生关键影响，特别是当它与富血小板血浆 (PRP) 结合时。该研究使用了 36 只白化小鼠 (BALB/c)，将它们分为阴性对照、PRP 治疗、BMAC 治疗和 BMAC 加 PRP 治疗。在小鼠背部切开伤口（1 cm 2 ），并根据治疗计划和分组分配对其伤口进行治疗。随后，在第7天、第14天和第21天收集治疗伤口部位的皮肤进行组织病理学检查。结果表明，在整个实验过程中，通过增加伤口收缩率、上皮再生过程和肉芽组织强度，BMAC+PRP治疗的伤口与其他治疗组相比，在加速伤口愈合方面存在统计学上的显着差异。与阴性对照、仅 PRP 和 BMAC 治疗组相比，中性粒细胞、巨噬细胞和淋巴细胞浸润数量波动，表皮和真皮厚度恢复，疤痕和毛囊再生更少。我们的研究结果表明，含有 MSC 的 BMAC 是一种有效的方法，可用于增强平滑的病理生理愈合过程，特别是与 PRP 结合使用时。

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Combinatorial influence of BMAC (Bone Marrow Aspirate Concentrate) and PRP (Platelet Rich Plasma) Treatment on Cutaneous Wound Healing in BALB/c Mice

Bone marrow, a soft spongy tissue, is containing mesenchymal stem cells (MSCs), that are well-recognized according to their self-renewability and stemness. Therefore, we hypothesized that BMAC could have a pivotal influence on the process of wound healing in particular when it is combined with platelet rich plasma (PRP). Thirty-six albino mice (BALB/c) were used in the study and they were grouped as negative-control, PRP treated, BMAC treated and BMAC plus PRP treated. An incisional wound (1 cm 2) was made at the back of mouse and their wounds were treated according to their treatment plan and group allocations. Later, the skin at the treated wound sites were collected on day-7, day-14, and day-21 for histopathological investigation. The results showed that there was a statistically significant difference in BMAC+PRP-treated wounds over the rest of the treated groups in the acceleration of wound healing throughout the experiment by increasing the rate of wound contraction, re-epithelization process, and granulation tissue intensity with fluctuated infiltration in the number of the neutrophils, macrophages, and lymphocytes, also restoration of the epidermal and dermal thickness with less scarring and hair follicle regeneration vs. to the negative-control, PRP and BMAC only treated groups. Our findings indicated that BMAC containing MSCs is an efficient approach, which can be used to enhance a smooth and physiopathological healing process, especially when it is used in combination with PRP.

更新日期：2023-06-02

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