Herein, a multi-mode assay platform was constructed for the detection of D-penicillamine based on perovskite nanocrystals (CsPbBr₃ NCs) and MnO₂ nanosheets. The water-dispersible CsPbBr₃ NCs synthesized via aqueous emulsion process has a strong fluorescence emission at 520 nm. The electrostatic interaction between positively charged CsPbBr₃ NCs and negatively charged MnO₂ nanosheets would lead to the fluorescence quenching of CsPbBr₃ NCs on account of the inner filter effect (IFE). The addition of D-penicillamine could convert MnO₂ nanosheets to Mn²⁺ and weaken inner filter effect, resulting in distinct recovery of fluorescence at 520 nm and the decrease of the absorption of CsPbBr₃ NCs/MnO₂ at 375 nm. Therefore, the fluorescence and colorimetric assay system for D-penicillamine was developed. The fluorescence and colorimetric sensing system could detect D-penicillamine ranging from 0.1 to 40 µM and 0.5 to 40 µM with the limits of detection (LOD) were 0.061 µM and 0.24 µM, respectively. The developed assay system could be applied to analyze D-penicillamine in real samples and acquired satisfactory results. Furthermore, a portable intelligent detection device was developed based on the mobile phone and RGB analysis software, demonstrating the practicability and potential of the sensing system.

_{在此，构建了基于钙钛矿纳米晶体（CsPbBr 3} NCs）和MnO ₂纳米片的多模式检测平台，用于检测D-青霉胺。通过水乳液法合成的水分散性 CsPbBr _{3 NCs 在 520 nm 处具有很强的荧光发射。}带正电的 CsPbBr ₃ NCs 和带负电的 MnO ₂纳米片之间的静电相互作用将导致 CsPbBr ₃ NCs 的荧光猝灭，这是由于内部过滤效应 (IFE)。D-青霉胺的加入可以将MnO ₂纳米片转化为Mn ²⁺_{并减弱内滤光效应，导致520 nm处荧光明显恢复，CsPbBr 3} NCs/MnO ₂在375 nm处吸收减少。为此，开发了D-青霉胺的荧光比色测定系统。荧光和比色传感系统可检测范围为0.1至40 µM和0.5至40 µM的D-青霉胺，检测限（LOD）分别为0.061 µM和0.24 µM。所开发的测定系统可用于实际样品中D-青霉胺的分析，取得了满意的结果。此外，还开发了基于手机和RGB分析软件的便携式智能检测装置，展示了传感系统的实用性和潜力。