<br>邻苯基苯酚通过 ROS 诱导的斑马鱼胚胎氧化应激对颅面软骨发育的毒性,Science of the Total Environment

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邻苯基苯酚通过 ROS 诱导的斑马鱼胚胎氧化应激对颅面软骨发育的毒性
Science of the Total Environment ( IF 8.2 ) Pub Date : 2023-06-01 , DOI: 10.1016/j.scitotenv.2023.164396
Xiaomei Chen ₁ , Jun Guo ₁ , Yong Huang ₂ , Zekun Li ₁ , Wei Yuan ₂ , Suwen Zeng ₂ , Hanyi Zhu ₁ , Yinliang Zhong ₁ , Weiying Lin ₂ , Huiqiang Lu ₂ , Jian Yang ₁

Affiliation

邻苯基苯酚（OPP）是一种常用的防腐剂和杀菌剂，对人类健康和环境有一定的威胁。OPP 的环境暴露可能会对动物和人类造成潜在的健康危害，需要评估 OPP 的发育毒性。因此，采用斑马鱼模型评价 OPP 的生态影响，斑马鱼颅面骨骼主要来源于颅神经嵴干细胞（NCCs）。在这项研究中，斑马鱼在受精后 10 至 80 小时（hpf）暴露于 1,2,4 mg/L OPP。我们的研究观察到，OPP 可导致颅面咽弓发育的早期疾病，并导致行为异常。此外，qPCR 和酶活性显示 OPP 暴露会诱导活性氧（ROS）的产生和氧化应激。增殖细胞核抗原（PCNA）表明 NCCs 的增殖减少。在 OPP 暴露下，与 NCCs 迁移、增殖和分化相关的基因的 mRNA 表达发生了显著变化。虾青素（AST）是一种广泛使用的抗氧化剂，可以部分挽救暴露于 OPP 的颅面软骨发育。结果显示斑马鱼的氧化应激、基因转录、NCCs 增殖和蛋白质表达有所改善，表明 OPP 可能降低抗氧化能力，从而抑制 NCCs 的迁移、增殖和分化。总之，我们的研究发现 OPP 可能产生活性氧，导致斑马鱼颅面软骨发育毒性。

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Toxicity of o-phenylphenol on craniofacial cartilage development through ROS-induced oxidative stress in zebrafish embryos

O-phenylphenol (OPP), a commonly used antiseptic and bactericide, has some threat to human health and the environment. Environmental exposure to OPP may cause potential health hazards in animals and humans, and the developmental toxicity of OPP needs to be assessed. Therefore, the zebrafish model was used to evaluate the ecological impact of OPP, and the zebrafish craniofacial skeleton is mainly derived from the cranial neural crest stem cells (NCCs). In this study, zebrafish were exposed to 1,2,4 mg/L OPP from10 to 80 h post-fertilization (hpf). Our study observed that OPP could cause the early disorder of craniofacial pharyngeal arch development and lead to behavioural abnormalities. In addition, qPCR and enzyme activity revealed that OPP exposure would induce the production of reactive oxygen species (ROS) and oxidative stress. And proliferation cell nuclear antigen (PCNA) indicated that the proliferation of NCCs was reduced. The mRNA expression of genes related to migration, proliferation, and differentiation of NCCs has changed significantly under OPP exposure. Astaxanthin (AST), a widely used antioxidant, could partially rescue the craniofacial cartilage development exposed to OPP. The results showed improvements in oxidative stress, gene transcription, NCCs proliferation, and protein expression in zebrafish, suggesting that OPP may reduce antioxidant capacity and subsequently inhibit migration, proliferation, and differentiation of the NCCs. In conclusion, our study found that OPP may produce reactive oxygen species, leading to developmental toxicity in zebrafish craniofacial cartilage.

更新日期：2023-06-01

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