Novel self-assembling cyclic peptides with reversible supramolecular nanostructures,Materials Chemistry Frontiers

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Novel self-assembling cyclic peptides with reversible supramolecular nanostructures
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2023-06-01 , DOI: 10.1039/d3qm00198a
Maria Gessica Ciulla ₁ , Federico Fontana _{1,

2} , Roberto Lorenzi ₃ , Amanda Marchini _{1,

2} , Luca Campone ₄ , Ehsan Sadeghi ₃ , Alberto Paleari ₃ , Sara Sattin ₅ , Fabrizio Gelain _{1,

2}

Affiliation

Self-assembly peptides (SAPs) are an important class of hydrogels used in nanomedicine for tissue repair and neural regeneration. Due to their unique properties, SAPs may be used in a wide range of applications but some limitations, such as low bioavailability and rapid hydrolysis degradation, need to be overcome. Here, we describe the synthesis and characterization of two novel cyclic SAPs without the use of D/L-alternating amino acids, showing a reversible transition of their supramolecular nanostructures, from nanotubes/nanofibers into nanovesicles/nanospheres. The investigation, characterization and optimization are performed using atomic force microscopy (AFM), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, Raman analysis, circular dichroism (CD), and rheology measurements. Also, in vitro cell viability assays show negligible toxicity of the representative optimized cyclic SAP towards human neural stem cells (hNSCs). Our results suggest that linear SAP theoretical background can be applied to develop cyclic SAPs, with important implications in the scalable fabrication of inter-changeable nanostructures, as well as for biomedical applications, including tissue regeneration, drug-delivery, drug-design, sensing, imaging, and size selectivity.

中文翻译：

具有可逆超分子纳米结构的新型自组装环肽

自组装肽 (SAP) 是纳米医学中用于组织修复和神经再生的一类重要水凝胶。由于其独特的性质，SAP 可用于广泛的应用，但需要克服一些限制，例如低生物利用度和快速水解降解。在这里，我们描述了两种不使用D / L的新型循环 SAP 的合成和表征-交替氨基酸，显示其超分子纳米结构的可逆转变，从纳米管/纳米纤维到纳米囊泡/纳米球。使用原子力显微镜 (AFM)、衰减全反射-傅里叶变换红外 (ATR-FTIR) 光谱、拉曼分析、圆二色性 (CD) 和流变学测量进行调查、表征和优化。还有，体外细胞活力测定显示代表性的优化循环 SAP 对人类神经干细胞 (hNSC) 的毒性可忽略不计。我们的结果表明，线性 SAP 理论背景可用于开发循环 SAP，对可互换纳米结构的可扩展制造以及生物医学应用（包括组织再生、药物输送、药物设计、传感、成像和尺寸选择性。

更新日期：2023-06-01

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