One of the main reasons impeding wound healing is wound infection caused by bacterial colonization with a continuous stage of inflammation. Traditional wound treatments like gauze are being replaced by tissue adhesives with strong wet tissue adhesion and biocompatibility. Herein, a fast-crosslinking hydrogel is developed to achieve both strong antimicrobial properties and excellent biocompatibility. In this study, a simple and non-toxic composite hydrogel was prepared by the Schiff base reaction between the aldehyde group of 2,3,4-trihydroxybenzaldehyde (TBA) and the amino group of ε-Poly-L-lysine (EPL). Subsequently, a succession of experiments toward this new hydrogel including structure characterization, antimicrobial properties, cell experiment and wound healing were applied. The results of the experiments show that the EPL-TBA hydrogel not only exhibited excellent contact-active antimicrobial activities against Gram-negative bacteria Escherichia coli (E. coil) and Gram-positive Bacteria Staphylococcus aureus (S. aureus), but also inhibited the biofilm formation. More importantly, the EPL-TBA hydrogel promoted the wound healing with low cytotoxicity in vivo. These findings indicate that the EPL-TBA hydrogel has a promising use as a wound dressing in the bacterial infection prevention and wounds healing acceleration.

中文翻译：

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一种快速交联的抗菌水凝胶，具有增强的抗菌能力，可改善伤口愈合

阻碍伤口愈合的主要原因之一是细菌定植引起的伤口感染，并伴有持续的炎症阶段。纱布等传统伤口治疗方法正在被具有强湿组织粘附力和生物相容性的组织粘合剂所取代。本文开发了一种快速交联水凝胶，以实现强大的抗菌特性和优异的生物相容性。本研究通过2,3,4-三羟基苯甲醛（TBA）的醛基与氨基的席夫碱反应制备了一种简单无毒的复合水凝胶。ε-聚-L-赖氨酸（EPL）。随后，针对这种新型水凝胶进行了一系列实验，包括结构表征、抗菌特性、细胞实验和伤口愈合。实验结果表明EPL-TBA水凝胶不仅对革兰氏阴性菌表现出优异的接触式抗菌活性大肠杆菌（E.线圈) 和革兰氏阳性细菌金黄色葡萄球菌（金黄色葡萄球菌），同时也抑制了生物膜的形成。更重要的是，EPL-TBA水凝胶促进伤口愈合且细胞毒性低体内。这些发现表明EPL-TBA水凝胶作为伤口敷料在预防细菌感染和加速伤口愈合方面具有广阔的应用前景。