当前位置：
X-MOL 学术
›
ACS Biomater. Sci. Eng.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Multifunctional Mitochondria-Targeting Near-Infrared Fluorescent Probe for Viscosity, ONOO–, Mitophagy, and Bioimaging
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2023-05-30 , DOI: 10.1021/acsbiomaterials.3c00307 Peng Lei 1 , Minglu Li 2 , Chuan Dong 3 , Shaomin Shuang 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2023-05-30 , DOI: 10.1021/acsbiomaterials.3c00307 Peng Lei 1 , Minglu Li 2 , Chuan Dong 3 , Shaomin Shuang 1
Affiliation
|
Irregularities in mitochondrial viscosity and peroxynitrite (ONOO–) concentration can lead to mitochondrial dysfunction. It is still a great challenge to develop near-infrared (NIR) fluorescent probes to simultaneously detect viscosity, endogenous ONOO–, and mitophagy. Herein, a multifunctional mitochondria-targeting NIR fluorescent probe P-1 was first synthesized for simultaneously detecting viscosity, ONOO–, and mitophagy. P-1 used quinoline cations as a mitochondrial targeting moiety, arylboronate as an ONOO– responsive group, and detected the change of viscosity by the twisted internal charge transfer (TICT) mechanism. The probe has an excellent response to the viscosity during inflammation by lipopolysaccharides (LPSs) and mitophagy induced by starvation at 670 nm. The viscosity changes of the probe induced by nystatin in zebrafish showed that P-1 was able to detect microviscosity in vivo. P-1 also showed good sensitivity with a detection limit of 6.2 nM for ONOO– detection and was successfully applied to the endogenous ONOO– detection in zebrafish. Moreover, P-1 has the ability to distinguish between cancer cells and normal cells. All of these features make P-1 a promising candidate to detect mitophagy and ONOO– -associated physiological and pathological processes.
中文翻译：
用于粘度、ONOO–、线粒体自噬和生物成像的多功能线粒体靶向近红外荧光探针
线粒体粘度和过氧亚硝酸盐 (ONOO – ) 浓度的不规则可导致线粒体功能障碍。开发近红外（NIR）荧光探针来同时检测粘度、内源性ONOO-和线粒体自噬仍然是一个巨大的挑战。在此，首先合成了一种多功能线粒体靶向近红外荧光探针P-1，用于同时检测粘度、ONOO –和线粒体自噬。P-1使用喹啉阳离子作为线粒体靶向部分，芳基硼酸盐作为ONOO响应基团，并通过扭曲内部电荷转移（TICT）机制检测粘度的变化。该探针对脂多糖 (LPS) 炎症期间的粘度和饥饿诱导的线粒体自噬在 670 nm 处具有出色的响应。制霉菌素在斑马鱼体内引起的探针粘度变化表明P-1能够检测体内的微粘度。P-1还表现出良好的灵敏度，ONOO 检测的检测限为 6.2 nM ，并成功应用于斑马鱼的内源性 ONOO 检测。而且，P-1具有区分癌细胞和正常细胞的能力。所有这些特征使P-1成为检测线粒体自噬和 ONOO 相关生理和病理过程的有前途的候选者。
更新日期：2023-05-30
中文翻译：
用于粘度、ONOO–、线粒体自噬和生物成像的多功能线粒体靶向近红外荧光探针
线粒体粘度和过氧亚硝酸盐 (ONOO – ) 浓度的不规则可导致线粒体功能障碍。开发近红外（NIR）荧光探针来同时检测粘度、内源性ONOO-和线粒体自噬仍然是一个巨大的挑战。在此，首先合成了一种多功能线粒体靶向近红外荧光探针P-1，用于同时检测粘度、ONOO –和线粒体自噬。P-1使用喹啉阳离子作为线粒体靶向部分，芳基硼酸盐作为ONOO响应基团，并通过扭曲内部电荷转移（TICT）机制检测粘度的变化。该探针对脂多糖 (LPS) 炎症期间的粘度和饥饿诱导的线粒体自噬在 670 nm 处具有出色的响应。制霉菌素在斑马鱼体内引起的探针粘度变化表明P-1能够检测体内的微粘度。P-1还表现出良好的灵敏度，ONOO 检测的检测限为 6.2 nM ，并成功应用于斑马鱼的内源性 ONOO 检测。而且，P-1具有区分癌细胞和正常细胞的能力。所有这些特征使P-1成为检测线粒体自噬和 ONOO 相关生理和病理过程的有前途的候选者。
京公网安备 11010802027423号