This study proposed fresh leachate treatment with anaerobic membrane bioreactor (AnMBR) based on the on-site investigation of the characteristics of fresh leachate. Temperature-related profiles of fresh leachate properties, like chemical oxygen demand (COD), were observed. In addition, AnMBR achieved a high COD removal of 98% with a maximum organic loading rate (OLR) of 19.27 kg-COD/m³/d at the shortest hydraulic retention time (HRT) of 1.5 d. The microbial analysis implied that the abundant protein and carbohydrate degraders (e.g., Thermovirga and Petrimonas) as well as syntrophic bacteria, such as Syntrophomonas, ensured the effective adaptation of AnMBR to the reduced HRTs. However, an excessive OLR at 36.55 kg-COD/m³/d at HRT of 1 d resulted in a sharp decrease in key microbes, such as archaea (from 37% to 15%), finally leading to the deterioration of AnMBR. This study provides scientific guidance for treating fresh leachate by AnMBR and its full-scale application for high-strength wastewater.

本研究在现场调查新鲜渗滤液特性的基础上，提出采用厌氧膜生物反应器（AnMBR）处理新鲜渗滤液。观察了新鲜渗滤液特性（如化学需氧量 (COD)）与温度相关的特征。此外，AnMBR实现了98%的高COD去除率，最大有机负荷率(OLR)为19.27 kg-COD/m ³ /d，最短水力停留时间(HRT)为1.5 d。微生物分析表明，丰富的蛋白质和碳水化合物降解剂（例如Thermovirga和Petrimonas）以及互养细菌（例如Syntropicomonas）确保AnMBR 有效适应降低的HRT。然而，在1天的HRT下，OLR过高，达到36.55 kg-COD/m ³ /d，导致古细菌等关键微生物急剧减少（从37%降至15%），最终导致AnMBR恶化。该研究为AnMBR处理新鲜渗滤液及其在高浓度废水中的全面应用提供了科学指导。