Mn₃O₄ nanoparticles composed of porous reduced graphene oxide nanosheets (Mn₃O₄@p-rGO) with enhanced oxidase-like activity were successfully fabricated through an in-situ approach for fast colorimetric detection of ascorbic acid (AA). The residual Mn²⁺ in the GO suspension of Hummers method was directly reused as the manganese source, improving the atom utilization efficiency. Benefiting from the uniform distribution of Mn₃O₄ nanoparticles on the surface of p-rGO nanosheets, the nanocomposite exhibited larger surface area, more active sites, and accelerated electron transfer efficiency, which enhanced the oxidase-like activity. Mn₃O₄@p-rGO nanocomposite efficiently activate dissolved O₂ to generate singlet oxygen (¹O₂), leading to high oxidation capacity toward the substrate 3,3′,5,5′–tetramethylbenzidine (TMB) without the extra addition of H₂O₂. Furthermore, the prominent absorption peak of the blue ox-TMB at 652 nm gradually decreased in the presence of AA, and a facile and fast colorimetric sensor was constructed with a good linear relationship (0.5–80 μM) and low LOD (0.278 μM) toward AA. Owing to the simplicity and excellent stability of the sensing platform, its practical application for AA detection in juices has shown good feasibility and reliability compared with HPLC and the 2, 4-dinitrophenylhydrazine colorimetric method. The oxidase-like Mn₃O₄@p-rGO provides a versatile platform for applications in food testing and disease diagnosis.

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