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Comprehensive Treatment Strategy for Banana Inflorescence Bract to Synthesize Biodiesel and Bioethanol Through Fungal Biorefinery
Waste and Biomass Valorization ( IF 2.6 ) Pub Date : 2023-05-28 , DOI: 10.1007/s12649-023-02166-9
Sundaram Deepika Bharathi , Samuel Jacob

Banana inflorescence bract (BIB), an agro-waste is sporadically explored for second-generation biofuel production in spite of having considerable holocellulosic composition (cellulose-35.56%, w/w; hemicellulose-22.41%, w/w). In this study, an attempt has been made to utilize this substrate for fermentable sugars (pentose-C5 and hexose-C6) extraction which were employed for the co-production of microbial lipids and ethanol using Rhodosporidium toruloides NCIM 3547 and Saccharomyces cerevisiae respectively. Since, a considerable amount of lignin (8.78%, w/w) is present in BIB, a hybrid pretreatment and carbohydrate hydrolysis through microwave (160 W) assisted mild H2SO4 acid 2.5% (v/v) was adopted. The resultant liquor contains holocellulosic sugars (C5 and C6 sugars), out of which xylose (10.40 ± 0.49 g/L) and glucose (51.48 ± 1.14 g/L). Hence, it was used as the growth medium for R. toruloides to produce lipids i.e., single cell oil (SCO). The maximum lipid content was found to be 44.89 ± 1.25 (%, w/w) containing total saturated fatty acids of 89.07% which justifies its potential application in biodiesel production. On the other hand, the pretreated solid fraction containing cellulose was saccharified using cellulolytic enzyme produced by Aspergillus sp. with saccharification (%) of 69.99 ± 0.30 (%, v/w) and yield of 27.22 ± 0.33 g/L of reducing sugar. This enzymatic hydrolysate was used for ethanol production by Saccharomyces cerevisiae resulting in an ethanol yield of 12.70 ± 0.09 g/L and productivity of 0.132 g/L/h. Based on this outcome, a sustainable route for agro-waste management of BIB was laid by favouring the integrated production of biodiesel and bioethanol towards a biorefinery approach.

Graphical Abstract



中文翻译:

香蕉花序苞片真菌生物炼制合成生物柴油和生物乙醇的综合处理策略

香蕉花序苞片 (BIB) 是一种农业废弃物,尽管具有相当多的全纤维素成分(纤维素 - 35.56%,w/w;半纤维素 - 22.41%,w/w),但仍被零星地探索用于第二代生物燃料生产。在这项研究中,尝试利用该底物提取可发酵糖(戊糖-C5 和己糖-C6),分别使用圆红冬孢菌NCIM 3547 和酿酒酵母联合生产微生物脂质和乙醇。由于 BIB 中存在大量木质素 (8.78%, w/w),混合预处理和碳水化合物水解通过微波 (160 W) 辅助温和的 H 2 SO 4采用 2.5% (v/v) 的酸。所得液体含有全纤维素糖(C5 和 C6 糖),其中木糖 (10.40 ± 0.49 g/L) 和葡萄糖 (51.48 ± 1.14 g/L)。因此,它被用作R. toruloides的生长培养基以产生脂质,即单细胞油 (SCO)。发现最大脂质含量为 44.89 ± 1.25 (%, w/w),其中总饱和脂肪酸为 89.07%,这证明了其在生物柴油生产中的潜在应用。另一方面,使用曲霉菌产生的纤维素分解酶将预处理后的含有纤维素的固体部分糖化。糖化率 (%) 为 69.99 ± 0.30 (%, v/w),还原糖产量为 27.22 ± 0.33 g/L。该酶促水解产物用于生产乙醇酿酒酵母的乙醇产量为 12.70 ± 0.09 g/L,生产率为 0.132 g/L/h。基于这一结果,通过支持生物柴油和生物乙醇的综合生产转向生物精炼方法,为 BIB 的农业废物管理奠定了可持续的途径。

图形概要

更新日期:2023-05-28
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