Aggregation-induced emission artificial enzyme (AIEzyme) with DNase-like activity: Imaging and matrix cleavage for combating bacterial biofilm,Aggregate

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Aggregation-induced emission artificial enzyme (AIEzyme) with DNase-like activity: Imaging and matrix cleavage for combating bacterial biofilm
Aggregate ( IF 13.9 ) Pub Date : 2023-05-25 , DOI: 10.1002/agt2.360
Lei Han ₁ , Yucui Zhang ₁ , Baojian Huang ₁ , Xuhui Bian ₁ , Ben Zhong Tang ₂

Affiliation

DNase-catalyzed hydrolysis of extracellular DNA (eDNA) has been widely employed to eradicate intractable biofilms. Although aggregation-induced emission (AIE) has become the ideal tool for killing planktonic bacteria, AIE luminogens (AIEgens) often lack DNase-mimetic activity, so as to suffer from poor anti-biofilm capacity. Here, an “AIEzyme”, a kind of AIE nanomaterial with enzyme-like activity, is designed and synthesized, where the AIEgens are used as the ligands of Zr-based coordination polymer nanoparticles. Not only does AIEzyme have enduring DNase-mimetic activity with low activation energy, but also structural rigidity-stabilized fluorescence. Due to the long-acting hydrolysis for eDNA in biofilm, AIEzyme can efficiently disorganize the established biofilms with good penetrability and realize the healing of superbug-infected wounds under only one dose of AIEzyme. This work provides a strategy to endow ordinary AIEgens with DNase-like and anti-biofilm activities. Moreover, AIEzymes can be observed by virtue of their own AIE character, facilitating the study on self-positioning and residual amount of AIEzymes in wounds. The concept “AIEzyme” would hopefully stimulate the tremendous expansion for the tool kits and the application of AIEgens and artificial enzymes.

中文翻译：

具有类似 DNase 活性的聚集诱导发射人工酶 (AIEzyme)：用于对抗细菌生物膜的成像和基质裂解

DNase 催化的细胞外 DNA (eDNA) 水解已被广泛用于根除棘手的生物膜。尽管聚集诱导发射（AIE）已成为杀灭浮游细菌的理想工具，但AIE发光剂（AIEgens）往往缺乏DNase模拟活性，因而抗生物膜能力较差。在此，设计并合成了一种具有类酶活性的AIE纳米材料“AIEzyme”，其中AIEgens被用作Zr基配位聚合物纳米颗粒的配体。AIEzyme 不仅具有低活化能、持久的 DNase 模拟活性，而且具有结构刚性稳定的荧光。由于AIEzyme对生物膜中eDNA的长效水解作用，可以高效地瓦解已形成的生物膜，且具有良好的渗透性，只需一剂AIEzyme即可实现超级细菌感染伤口的愈合。这项工作提供了一种赋予普通 AIEgen 类 DNase 和抗生物膜活性的策略。而且，AIEzymes可以凭借其自身的AIE特性进行观察，有利于研究AIEzymes在伤口中的自我定位和残留量。“AIEzyme”的概念有望刺激工具包的巨大扩展以及AIEgens和人工酶的应用。

更新日期：2023-05-25

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