CRP 蛋白对细菌环核苷酸感知的结构和功能多样性。,microLife

当前位置： X-MOL 学术 › › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

CRP 蛋白对细菌环核苷酸感知的结构和功能多样性。
microLife Pub Date : 2023-05-01 , DOI: 10.1093/femsml/uqad024
Elizaveta Krol _{1,

2} , Laura Werel ₃ , Lars Oliver Essen ₃ , Anke Becker _{1,

2}

Affiliation

环 AMP (cAMP) 是一种普遍存在的第二信使，由大多数生物体合成。在细菌中，它在新陈代谢、宿主定植、运动和许多其他对最佳适应性很重要的过程中发挥着高度多样化的作用。cAMP 感知的主要途径是通过来自多种多样的 CRP-FNR 蛋白超家族的转录因子。自从四十多年前在大肠杆菌中发现第一个 CRP 蛋白 CAP 以来，其同系物已在密切相关和遥远的细菌物种中得到表征。在没有葡萄糖的情况下，cAMP 介导的 CRP 蛋白碳分解代谢基因激活似乎仅限于大肠杆菌及其近亲。在其他门中，监管目标更加多样化。除 cAMP 外，cGMP 最近已被确定为某些 CRP 蛋白的配体。在 CRP 二聚体中，两个环核苷酸分子中的每一个都与两个蛋白质亚基接触并实现有利于 DNA 结合的构象变化。在这里，我们总结了大肠杆菌 CAP 与其他 cAMP 和 cGMP 激活的转录因子相比的结构和生理方面的当前知识，并指出了与 CRP 蛋白的赖氨酸修饰和膜结合相关的代谢调节的新趋势。

"点击查看英文标题和摘要"

Structural and functional diversity of bacterial cyclic nucleotide perception by CRP proteins.

Cyclic AMP (cAMP) is a ubiquitous second messenger synthesized by most living organisms. In bacteria, it plays highly diverse roles in metabolism, host colonization, motility, and many other processes important for optimal fitness. The main route of cAMP perception is through transcription factors from the diverse and versatile CRP-FNR protein superfamily. Since the discovery of the very first CRP protein CAP in Escherichia coli more than four decades ago, its homologs have been characterized in both closely related and distant bacterial species. The cAMP-mediated gene activation for carbon catabolism by a CRP protein in the absence of glucose seems to be restricted to E. coli and its close relatives. In other phyla, the regulatory targets are more diverse. In addition to cAMP, cGMP has recently been identified as a ligand of certain CRP proteins. In a CRP dimer, each of the two cyclic nucleotide molecules makes contacts with both protein subunits and effectuates a conformational change that favors DNA binding. Here, we summarize the current knowledge on structural and physiological aspects of E. coli CAP compared with other cAMP- and cGMP-activated transcription factors, and point to emerging trends in metabolic regulation related to lysine modification and membrane association of CRP proteins.

更新日期：2023-05-01

点击分享查看原文

点击收藏

公开下载