基于血小板膜的仿生基质囊泡用于牙本质小管的生物矿化,Industrial & Engineering Chemistry Research

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基于血小板膜的仿生基质囊泡用于牙本质小管的生物矿化
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2023-05-24 , DOI: 10.1021/acs.iecr.3c00188
Shijie Shi _{1,

2} , Haiqin Tang ₃ , Li Zhen _{1,

2} , Menglin Fan _{1,

2} , Lizhong Sun _{1,

2} , Siying Tao _{1,

2} , Jiaojiao Yang ₁ , Jianshu Li _{1,

3,

4} , Jiyao Li _{1,

2}

Affiliation

基质囊泡（Matrix vesicles，MVs）是细胞分泌的一种细胞外囊泡，在硬组织矿化的初期起着重要作用。嵌入 MV 膜表面的酸性磷脂和功能性膜蛋白提供矿物成核位点并调节矿物离子以促进生物矿化。在这里，我们报告了由天然血小板膜碎片制备的 MVs 模拟纳米囊泡。由于其稳定的纳米结构，受生物启发的纳米囊泡可以渗透到深层牙本质小管中，并为新生矿物晶体提供成核位点和模板。同时，纳米囊泡表面的酸性磷脂可以从环境中吸收矿物离子，有效促进原位生物矿化。另一方面，纳米囊泡表面的蛋白质可以促进胶原蛋白交联，抑制胶原蛋白的酶解，进一步保护这种矿化模板。新形成的矿物质能有效封闭牙本质小管，封闭深度可达60μm。该策略利用源自血小板膜的“人工MVs”进行牙本质的生物矿化，为临床实践中的硬组织修复带来了新的前景。

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Bioinspired Matrix Vesicles Based on Platelet Membrane for Biomineralization of Dentin Tubules

Matrix vesicles (MVs), a kind of extracellular vesicles secreted by cells, play an important role in the initial stage of hard tissue mineralization. Acidic phospholipids and functional membrane proteins which are embedded into the membrane surface of MVs provide mineral nucleation sites and regulate mineral ions for facilitating biomineralization. Here, we report the MVs-mimic nanovesicles prepared from natural platelet membrane fragments. The bioinspired nanovesicles can penetrate into the deep dentinal tubule due to their stable nanostructure, and provide nucleation sites and templates for nascent mineral crystals. Meanwhile, the acidic phospholipids on the nanovesicle surface can recruit mineral ions from the environment to effectively promote biomineralization in situ. On the other hand, proteins on the surface of the nanovesicles can promote collagen cross-linking to inhibit the enzymatic hydrolysis of collagen, and further protect this mineralization template. The newly formed minerals can effectively seal the dentinal tubules, and even the sealing depth can reach 60 μm. This strategy uses “artificial MVs” derived from platelet membranes for the biomineralization of dentin, bringing new prospects for hard tissue repair in clinical practice.

更新日期：2023-05-24

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