Indole-3-carbinol (I3C) is a type of natural anticancer while the low extraction efficiency of I3C limits its application. In this study, a novel two-dimensional metal-organic framework Cu-TCPP @AuNPs-MPB was synthesized by chemical precipitation and in situ polymerization, used for enrich I3C in cruciferous vegetables. The synthesized material was analyzed by Scanning electron microscopy (SEM), X-ray diffraction (XRD), Element analysis (EA), Inductively coupled plasma (ICP), Fourier transform infrared spectroscopy (FT-IR), Brunauer-Emmett-Teller (BET) and X-ray photoelectron spectroscopy (XPS). Cu-TCPP@AuNPs-MPB loaded with MPB realized specific adsorption of I3C, a large number of properties and easily dispersed in aqueous solution, which provided more enrichment sites for I3C and greatly improved the efficiency of enrichment. It was found that 93% 200 μg/mL I3C could be enriched by per 20 mg Cu-TCPP@AuNPs-MPB within 30 min under optimal conditions. Combined with HPLC-MS/MS quantitative detection technology, a complete set of enrichment detection system has been constructed to enrich I3C in cruciferous vegetables. The whole process with high efficiency avoids the use of toxic solvents, which is environmental friendly, providing a new reference for production applications in the I3C industry.

Indole-3-carbinol (I3C) 是一种天然抗癌剂，但 I3C 的低提取效率限制了其应用。在这项研究中，通过化学沉淀和原位聚合合成了一种新型二维金属有机骨架 Cu-TCPP @AuNPs-MPB，用于富集十字花科蔬菜中的 I3C。通过扫描电子显微镜 (SEM)、X 射线衍射 (XRD)、元素分析 (EA)、电感耦合等离子体 (ICP)、傅里叶变换红外光谱 (FT-IR)、Brunauer-Emmett-Teller ( BET) 和 X 射线光电子能谱 (XPS)。负载MPB的Cu-TCPP@AuNPs-MPB实现了对I3C的特异性吸附，大量的性质和易分散在水溶液中，为I3C提供了更多的富集位点，大大提高了富集效率。结果发现，在最佳条件下，每 20 mg Cu-TCPP@AuNPs-MPB 可在 30 分钟内富集 93% 200 μg/mL I3C。结合HPLC-MS/MS定量检测技术，构建了一套完整的富集检测体系，富集了十字花科蔬菜中的I3C。整个过程效率高，避免使用有毒溶剂，对环境友好，为I3C行业的生产应用提供了新的参考。