Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A fluorescent probe for lipid droplet polarity imaging with low viscosity crosstalk
Analyst ( IF 3.6 ) Pub Date : 2023-05-23 , DOI: 10.1039/d3an00551h Bo Lin 1 , Zhenru Li 1 , Qi Zan 2 , Li Fan 2 , Yang Shu 1 , Jianhua Wang 1
Analyst ( IF 3.6 ) Pub Date : 2023-05-23 , DOI: 10.1039/d3an00551h Bo Lin 1 , Zhenru Li 1 , Qi Zan 2 , Li Fan 2 , Yang Shu 1 , Jianhua Wang 1
Affiliation
|
Monitoring the variations of lipid droplet (LD) polarity is of great significance for the investigation of LD-related cellular metabolism and function. We hereby report a lipophilic fluorescent probe (BTHO) with the feature of intramolecular charge transfer (ICT) for imaging the LD polarity in living cells. BTHO exhibits an obvious attenuation of fluorescence emission in response to the increase of environmental polarity. The linear response range of BTHO to polarity (ε, the dielectric constant of solvents) is derived to be 2.21–24.40, and the fluorescence of BTHO in glyceryl trioleate falls in this range. Furthermore, BTHO has high molecular brightness, which may effectively improve the signal to noise ratio, along with the decrease of phototoxicity. BTHO exhibits excellent photostability and targeting capability to LDs with low cytotoxicity, which is satisfactory in long-term imaging in live cells. The probe was successfully applied for imaging LD polarity variation in live cells caused by oleic acid (OA), methyl-β-cyclodextrin (MβCD), H2O2, starvation, lipopolysaccharide (LPS), nystatin, and erastin. The low crosstalk caused by viscosity to BTHO measuring the LD polarity was confirmed from a calculation result.
中文翻译：
低粘度串扰脂滴极性成像荧光探针
监测脂滴（LD）极性的变化对于研究LD相关的细胞代谢和功能具有重要意义。我们在此报道了一种具有分子内电荷转移（ICT）特征的亲脂性荧光探针（BTHO），用于对活细胞中的LD极性进行成像。BTHO随着环境极性的增加而表现出明显的荧光发射衰减。BTHO 对极性的线性响应范围 ( ε，溶剂的介电常数）得出为2.21-24.40，三油酸甘油酯中BTHO的荧光落在这个范围内。此外，BTHO具有较高的分子亮度，可以有效提高信噪比，同时降低光毒性。BTHO表现出优异的光稳定性和低细胞毒性的LD靶向能力，这在活细胞的长期成像中是令人满意的。该探针成功应用于对油酸（OA）、甲基-β-环糊精（MβCD）、H 2 O 2 、饥饿、脂多糖（LPS）、制霉菌素和erastin引起的活细胞LD极性变化进行成像。计算结果证实了测量 LD 极性的 BTHO 的粘度引起的低串扰。
更新日期：2023-05-23
中文翻译：
低粘度串扰脂滴极性成像荧光探针
监测脂滴（LD）极性的变化对于研究LD相关的细胞代谢和功能具有重要意义。我们在此报道了一种具有分子内电荷转移（ICT）特征的亲脂性荧光探针（BTHO），用于对活细胞中的LD极性进行成像。BTHO随着环境极性的增加而表现出明显的荧光发射衰减。BTHO 对极性的线性响应范围 ( ε，溶剂的介电常数）得出为2.21-24.40，三油酸甘油酯中BTHO的荧光落在这个范围内。此外，BTHO具有较高的分子亮度，可以有效提高信噪比，同时降低光毒性。BTHO表现出优异的光稳定性和低细胞毒性的LD靶向能力，这在活细胞的长期成像中是令人满意的。该探针成功应用于对油酸（OA）、甲基-β-环糊精（MβCD）、H 2 O 2 、饥饿、脂多糖（LPS）、制霉菌素和erastin引起的活细胞LD极性变化进行成像。计算结果证实了测量 LD 极性的 BTHO 的粘度引起的低串扰。
京公网安备 11010802027423号