Abstract

Green microalgae capable of accumulating secondary carotenoids are the most important objects of biotechnology, and the search for new strains with unique properties, in particular, those adapted to growth at low temperatures and high salinity in the environment, is an urgent task. The NAMSU SBB-20 microalga strain was isolated from an algal-bacterial biofilm found on the coast of the White Sea in the littoral zone of the Solovetsky Archipelago. Identification of the strain showed its belonging to the species Halochlorella rubescens P.J.L.Dangeard. The species H. rubescens was first described for the White Sea. Under conditions of high light intensity, ultrastructural changes in cells are shown, among which destruction of the photosynthetic apparatus and the formation of cytoplasmic and chloroplast lipid inclusions are noted. It was shown that the culture of the NAMSU SBB-20 strain is capable of acquiring an orange color under unfavorable growth conditions. An assessment was made of the effect of the composition of the medium and the intensity of illumination on the pigment composition of the algae. The highest absolute values of the accumulation of carotenoids were noted during cultivation in light with an intensity of 150 μmol PAR quanta/m²/s on BG-11 media containing no source of phosphorus (15.66 ± 0.18 mg/L) or nitrogen (15.95 ± 0.56 mg/L). The described strain has a biotechnological potential due to the initial halotolerance and the accumulation of high values of secondary carotenoids in the biomass.

中文翻译：

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产类胡萝卜素微藻 Halochlorella rubescens NAMSU SBB-20 耐盐性北极新菌株的特性

摘要

能够积累次级类胡萝卜素的绿色微藻是生物技术最重要的对象，寻找具有独特特性的新菌株，特别是适应低温和高盐度环境生长的菌株，是一项紧迫的任务。NAMSU SBB-20 微藻菌株是从索洛维茨基群岛沿岸地区白海沿岸发现的藻类细菌生物膜中分离出来的。该菌株的鉴定表明其属于Halochlorella rubescens PJLDangeard种。H. rubescens物种第一次被描述为白海。在高光强度条件下，显示细胞的超微结构变化，其中注意到光合装置的破坏以及细胞质和叶绿体脂质内含物的形成。结果表明，NAMSU SBB-20 菌株的培养物能够在不利的生长条件下获得橙色。对培养基组成和光照强度对藻类色素组成的影响进行了评估。在强度为 150 μmol PAR 量子/m ^{2的光照下培养期间，注意到类胡萝卜素积累的最高绝对值}/s 在不含磷源 (15.66 ± 0.18 mg/L) 或氮源 (15.95 ± 0.56 mg/L) 的 BG-11 培养基上。由于最初的耐盐性和生物质中次级类胡萝卜素的高价值积累，所描述的菌株具有生物技术潜力。