Chickpea is one of the sources of plant protein with a yield capacity of about 6 tons per hectare. Because of cultivation in rainfed lands this plant has a very low average yield in most countries of the world. However, in the autumn cultivation of chickpeas, the amount of production increases significantly which is due to the increase in the use of winter rainfall. Nowadays, the introduction of cold tolerant cultivars and the identification of genotypes have provided the conditions for welcoming the chickpea winter cultivations. The main limiting factor for autumn cultivation of chickpeas is the increasing damage caused by Ascochyta blight (AB) due to the availability of suitable humidity and temperature for the growth of the fungus Ascochyta rabiei. The main aim of the present study was to determine the resistance pattern of selected MCC741 line and cold tolerant cultivar Saral against three pathotypes of A. rabiei named PI, PIII and PVI. For this purpose, a pathogenicity test method was designed and the effectiveness of this method was evaluated on different chickpea genotypes with different reported resistance levels and also by the pathogenicity test in green house. The results showed that the in vitro pathogenicity test method is well able to differentiate resistant and susceptible cultivars based on the symptoms of the disease. Furthermore, the virulence of the aforementioned pathotypes can be distinguished from each other. These results showed that this method can be used as a nondestructive method in prescreening studies and evaluation of resistance levels of a unique plant genotypes. This method is also recommended for pathotyping of new fungal isolates. In vitro assay on the resistance pattern of cold tolerant chickpea accessions showed that the Saral cultivar presented an appropriate resistance against pathotypes I and III but did not show a resistance against pathotype VI. The resistant pattern was confirmed by greenhouse pathogenicity test. It is necessary to evaluate more cold tolerant chickpea samples in other studies to identify sources of resistance to this pathotype.

鹰嘴豆是植物蛋白的来源之一，每公顷产量约为 6 吨。由于在雨养土地上种植，这种植物在世界上大多数国家的平均产量非常低。然而，在鹰嘴豆的秋季栽培中，由于冬季降雨的利用增加，产量显着增加。如今，耐寒品种的引进和基因型的鉴定，为鹰嘴豆越冬栽培提供了条件。鹰嘴豆秋季栽培的主要限制因素是 Ascochyta 枯萎病 (AB) 造成的损害增加，这是由于为真菌Ascochyta rabiei的生长提供了合适的湿度和温度. 本研究的主要目的是确定所选 MCC741 品系和耐寒品种 Saral 对三种致病型狂犬病的抗性模式命名为PI、PIII和PVI。为此，设计了一种致病性测试方法，并通过具有不同抗性报告水平的不同鹰嘴豆基因型和温室致病性测试评估了该方法的有效性。结果表明，体外致病性试验方法能够很好地根据病害症状区分抗病品种和感病品种。此外，可以区分上述致病型的毒力。这些结果表明，该方法可以作为一种非破坏性方法用于预筛选研究和评估独特植物基因型的抗性水平。这种方法也被推荐用于新的真菌分离株的致病分型。对耐寒鹰嘴豆种质抗性模式的体外测定表明，Saral 品种对病原体 I 和 III 表现出适当的抗性，但没有表现出对病原体 VI 的抗性。通过温室致病性试验证实了耐药模式。有必要在其他研究中评估更耐寒的鹰嘴豆样品，以确定对该致病型的抗性来源。