Complete genome sequence of Acinetobacter indicus and identification of the hydrolases provides direct insights into phthalate ester degradation,Food Science and Biotechnology

当前位置： X-MOL 学术 › Food Sci. Biotechnol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Complete genome sequence of Acinetobacter indicus and identification of the hydrolases provides direct insights into phthalate ester degradation
Food Science and Biotechnology ( IF 2.4 ) Pub Date : 2024-01-01 , DOI: 10.1007/s10068-023-01334-w
Huiqin Huang ₁ , Youqiang Xu ₁ , Mengwei Lin ₁ , Xiuting Li _{1,

2} , Hua Zhu _{2,

3} , Kun Wang _{2,

3} , Baoguo Sun _{1,

2,

4}

Affiliation

Abstract

A strain designated Acinetobacter indicus WMB-7 with the ability to hydrolyze phthalate esters (PAEs) was isolated from the fermented grains of Baijiu. The genome of the strain was sequenced with a length of 3,256,420 bp and annotated with 3183 genes, of which 36 hydrolases encoding genes were identified. The hydrolases were analyzed by protein structure modeling and molecular docking, and 14 enzymes were docked to the ligand di-butyl phthalate with the catalytic active regions, and showed binding affinity. The 14 enzymes were expressed in E. coli and 5 of them showed the ability for PAEs hydrolysis. Enzyme GK020_RS15665 showed high efficiency for PAEs hydrolysis and could efficiently hydrolyze di-butyl phthalate under an initial concentration of 1000 mg/L with a half-life of 4.24 h. This work combined a series of methods for identifying PAEs hydrolases and offered a molecular basis for PAEs degradation of A. indicus strains from Baijiu.

中文翻译：

印度不动杆菌的完整基因组序列和水解酶的鉴定为邻苯二甲酸酯降解提供了直接的见解

抽象的

从白酒发酵谷物中分离出一株具有水解邻苯二甲酸酯（PAE）能力的菌株，命名为Acinetobacter indicus WMB-7。该菌株基因组测序长度为3,256,420 bp，注释了3183个基因，其中鉴定出36个水解酶编码基因。通过蛋白质结构建模和分子对接对水解酶进行分析，14种酶与具有催化活性区域的配体邻苯二甲酸二丁酯对接，并表现出结合亲和力。这14种酶在大肠杆菌中表达，其中5种显示出PAEs水解能力。酶GK020_RS15665对PAEs具有较高的水解效率，可以在初始浓度1000 mg/L的条件下高效水解邻苯二甲酸二丁酯，半衰期为4.24 h。该工作结合了一系列鉴定PAEs水解酶的方法，为A的PAEs降解提供了分子基础。来自白酒的indicus菌株。

更新日期：2024-01-05

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南