<br>高脂诱导肥胖小鼠抗性淀粉富米消化过程中的结构特性及其对肠道屏障功能的影响,Food Research International

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高脂诱导肥胖小鼠抗性淀粉富米消化过程中的结构特性及其对肠道屏障功能的影响
Food Research International ( IF 7.0 ) Pub Date : 2023-05-20 , DOI: 10.1016/j.foodres.2023.113011
Hye-Bin Lee ₁ , Ha Ram Kim ₂ , Min-Cheol Kang ₂ , Duyun Jeong ₃ , Hee-Don Choi ₂ , Jung Sun Hong ₂ , Ho-Young Park ₄

Affiliation

众所周知，膳食中添加不易消化的碳水化合物可以通过调节肠道微生物群来改善肠道环境并预防肥胖和炎症性疾病。在以前的工作中，我们建立了一种使用柠檬酸生产富含抗性淀粉（RS）的高直链淀粉大米（R-HAR）的方法。本研究旨在评估 R-HAR 消化过程中结构特征的变化及其对肠道健康的影响。采用三步体外消化发酵模型，然后分析体外消化过程中的 RS 含量、扫描电镜和支链长度分布。在 R-HAR 消化过程中，RS 含量增加，预计该结构对肠道菌群和肠道环境的影响更大。为了研究 R-HAR 对肠道健康的影响，在 HFD 诱导的小鼠中测定了其抗炎和肠道屏障完整性活性。摄入 R-HAR 抑制了 HFD 诱导的结肠缩短和炎症反应。此外，R-HAR 表现出肠道屏障保护活性，紧密连接蛋白水平增加。我们确定 R-HAR 可能是一种可能有益的肠道环境改善剂，它可能在食品工业中与大米一样具有各种影响。

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Structural characteristics of resistant starch-enriched rice during digestion and its effects on gut barrier function in high-fat induced obese mice

Dietary supplementation with indigestible carbohydrates is known to improve the gut environment and prevent obesity and inflammatory diseases by modulating the gut microbiota. In previous work, we established a method for the production of resistant starch (RS)-enriched high-amylose rice (R-HAR) using citric acid. The present study aimed to evaluate changes in structural characteristics during digestion of R-HAR and its effects on the gut health. A three-step in vitro digestion and fermentation model was used, then, RS content, scanning electron microscopy, and branch chain length distribution were analyzed during in vitro digestion. During the digestion of R-HAR, the RS content increased, and the structure was predicted to have a greater impact on the gut microbiota and gut environment. To study the intestinal health effects of R-HAR, its anti-inflammatory and gut barrier integrity activities were assayed in HFD-induced mice. Intake of R-HAR suppressed colonic shortening and inflammatory responses induced by HFD. Furthermore, R-HAR exhibited gut barrier protective activity with an increase in tight junction protein levels. We determined that R-HAR may be a potentially beneficial intestinal environment improver, which may have various implications in the food industry as rice.

更新日期：2023-05-20

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