DNAmPhenoAge, DNAmGrimAge, and the newly developed DNAmFitAge are DNA methylation (DNAm)-based biomarkers that reflect the individual aging process. Here, we examine the relationship between physical fitness and DNAm-based biomarkers in adults aged 33–88 with a wide range of physical fitness (including athletes with long-term training history). Higher levels of VO₂max (ρ = 0.2, p = 6.4E − 4, r = 0.19, p = 1.2E − 3), Jumpmax (p = 0.11, p = 5.5E − 2, r = 0.13, p = 2.8E − 2), Gripmax (ρ = 0.17, p = 3.5E − 3, r = 0.16, p = 5.6E − 3), and HDL levels (ρ = 0.18, p = 1.95E − 3, r = 0.19, p = 1.1E − 3) are associated with better verbal short-term memory. In addition, verbal short-term memory is associated with decelerated aging assessed with the new DNAm biomarker FitAgeAcceleration (ρ: − 0.18, p = 0.0017). DNAmFitAge can distinguish high-fitness individuals from low/medium-fitness individuals better than existing DNAm biomarkers and estimates a younger biological age in the high-fit males and females (1.5 and 2.0 years younger, respectively). Our research shows that regular physical exercise contributes to observable physiological and methylation differences which are beneficial to the aging process. DNAmFitAge has now emerged as a new biological marker of quality of life.

DNAmPhenoAge、DNAmGrimAge 和新开发的 DNAmFitAge 是基于 DNA 甲基化 (DNAm) 的生物标志物，反映个体衰老过程。在这里，我们研究了 33-88 岁的成年人（包括具有长期训练历史的运动员）的身体素质和基于 DNAm 的生物标志物之间的关系。更高水平的 VO ₂ max ( ρ  = 0.2, p  = 6.4E − 4, r  = 0.19, p  = 1.2E − 3), Jumpmax ( p  = 0.11, p  = 5.5E − 2, r  = 0.13, p  = 2.8 E − 2)、Gripmax ( ρ  = 0.17、p  = 3.5E − 3、r  = 0.16、p  = 5.6E − 3) 和 HDL 水平 ( ρ  = 0.18、p  = 1.95E − 3、r  = 0.19、p  = 1.1E − 3) 与更好的言语短期记忆相关。此外，通过新的 DNAm 生物标志物 FitAgeAcceleration 评估，言语短期记忆与延缓衰老相关（ρ：− 0.18，p  = 0.0017）。DNAmFitAge 可以比现有的 DNAm 生物标志物更好地区分高适应度个体和低/中等适应度个体，并估计高适应度男性和女性的更年轻的生物学年龄（分别年轻 1.5 岁和 2.0 岁）。我们的研究表明，定期体育锻炼有助于观察到的生理和甲基化差异，这有利于衰老过程。DNAmFitAge 现在已成为生活质量的新生物标志物。