<br>通过 DNAzyme walker 集成纳米探针的双模型光电化学生物传感器，用于 microRNA-155 的超灵敏比例检测,Sensors and Actuators B: Chemical

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通过 DNAzyme walker 集成纳米探针的双模型光电化学生物传感器，用于 microRNA-155 的超灵敏比例检测
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2023-05-19 , DOI: 10.1016/j.snb.2023.133993
Liang Tian , Jiyuan Zhang , Yanci Zhang , Huiling Fan , Chun Liu , Yihong Wang , Jiansheng Cui

癌症早期血清中低浓度和异常表达的microRNA-155（miR-155）（fM水平）的超灵敏检测仍然存在很大的挑战。因此，基于无标记三金属纳米探针和镁依赖性DNA酶驱动的DNA步行器开发了双模型光电化学生物传感器，以产生比率双信号输出，用于对乳腺癌细胞（MCF-7）和宫颈癌的miR-155进行灵敏的生物传感细胞（海拉）。在信号放大策略中，选择PtCo@普鲁士蓝纳米酶作为具有高催化活性和大比表面积的信号纳米探针，同时采用二维MXene/AuNPs作为表面修饰材料来改善电极的活性位点。随着靶标的存在，DNAzyme 辅助 DNA walker 过程启动，并将丰富的信号纳米探针引入电极表面，产生高电流响应和弱紫外-可见分光光度 (UV-vis) 响应。因此，miR-155 生物传感器在 5 fM 至 5 nM 的线性范围内显示出 1.2 fM 的检测下限，表现出优异的稳定性和重现性。此外，所提出的生物传感器在复杂的人血清样本和肿瘤细胞裂解物中表现出miR-155的优越性能，为早期临床诊断提供了巨大的应用潜力。

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Dual-model photoelectrochemical biosensor via DNAzyme walker integrated nanoprobe for ultrasensitive ratiometric detection of microRNA-155

Ultrasensitive detection for low concentration and abnormal expression of microRNA-155 (miR-155) in serum (fM level) at the early stage of cancer still exist great challenges. Therefore, a dual-model photoelectrochemical biosensor was developed based on label-free trimetallic nanoprobe and Mg dependent DNAzyme driven DNA walker to generate ratiometric dual-signal output for sensitive biosensing of miR-155 from breast cancer cells (MCF-7) and cervical cancer cells (HeLa). In signal amplification strategy, PtCo@Prussian Blue nanozyme was selected as a signal nanoprobe with high catalytic activity and large specific surface area, while two dimensional MXene/AuNPs as surface modification material was applied to improve the active sites of the electrode. With the presence of the target, the DNAzyme assisted DNA walker process was initiated and abundant signal nanoprobe was introduced to the electrode surface, producing high current response and weak ultraviolet-visible spectrophotometry (UV–vis) response. Hence, the biosensor for miR-155 shows a low limit of detection of 1.2 fM from the linear range of 5 fM to 5 nM, exhibiting excellent stability and reproducibility. Moreover, the proposed biosensor demonstrates superior performance for miR-155 in complex human serum samples and tumor cell lysates, presenting great application potential for early-stage clinical diagnosis.

更新日期：2023-05-19

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