A novel dansyl-based fluorescent probe (DG) was designed via the introduction of a dipeptide, glycyl-L-glutamine. DG showed good selectivity and sensitivity towards Cu²⁺ in aqueous solutions in the pH span of ~ 6–12. The coordination of Cu²⁺ with the dipeptide moiety led to the fluorescent quenching of the dansyl fluorophore. The association constant value for Cu²⁺ was 0.78 × 10⁴ M^− 1 in a 1 to 1 stoichiometric ratio. The detection limit in HEPES buffer solution (10 mM, pH 7.4) was 1.52 µM. DG also showed strong anti-interference capability in the presence of other metal ions. It was worth noting that DG maintained the detection ability towards Cu²⁺ in real water samples and cell imaging, implying the potential application opportunities in complicated environments.

通过引入二肽甘氨酰-L-谷氨酰胺设计了一种新型基于丹酰基的荧光探针（DG）。DG对 pH 范围约为 6-12 的水溶液中的Cu ²⁺显示出良好的选择性和灵敏度。^{Cu 2+}与二肽部分的配位导致丹磺酰荧光团的荧光猝灭。^{Cu 2+}的缔合常数值为0.78×10 ⁴  M ^{- 1}，化学计量比为1:1。HEPES 缓冲溶液（10 mM，pH 7.4）中的检测限为 1.52 µM。DG在其他金属离子存在的情况下也表现出很强的抗干扰能力。值得注意的是，DG在实际水样和细胞成像中保持了对Cu ²⁺的检测能力，暗示着在复杂环境中的潜在应用机会。